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Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway

  • Protocol
Antigen Processing and Presentation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 156))

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Abstract

Differentiated CD4+T-cells produce a restricted set of cytokines, allowing their subdivision into two discrete populations: T-helper 1 (Th1), characterized by secretion of interleukin 2 (IL-2) and interferon γ (IFN-γ); and Th2, selectively producing IL-4, IL-5, and IL-10 (1). Polarized subsets of antigen(Ag)- specific CD4+Th1 and Th2 cells can be induced in vivo by Ag priming, and their development is primarily influenced by the cytokine milieu during the initial phase of the immune response. Among cytokines, decisive roles are played by IL-12 and IL-4, driving T-cell responses toward the Th1 or Th2 phenotype, respectively (2, 3). The polarization in Th1 and Th2 cells is also influenced by several other factors, including non-major histocompatibility complex (MHC) genetic polymorphism (4-6; see Note 1), ligand-T-cell receptor (TCR) interaction (7,8), Ag dose (9,10), and mode of Ag administration (11; see Note 2 ).

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Author information

Authors and Affiliations

  1. Roche Milano Ricerche, Via Olgettina, 58, I-20132 Milano, Italy

    Francesca Galbiati

  2. Roche Milano Ricerche, Via Olgetina, 58, I-20132 Milano, Italy

    Luciano Adorini

Authors
  1. Francesca Galbiati
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  2. Luciano Adorini
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Editors and Affiliations

  1. University of Nebraska Medical Center, Omaha, NE

    Joyce C. Solheim

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© 2000 Humana Press Inc.

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Galbiati, F., Adorini, L. (2000). Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway. In: Solheim, J.C. (eds) Antigen Processing and Presentation Protocols. Methods in Molecular Biology, vol 156. Humana Press. https://doi.org/10.1385/1-59259-062-4:233

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  • DOI: https://doi.org/10.1385/1-59259-062-4:233

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-745-8

  • Online ISBN: 978-1-59259-062-9

  • eBook Packages: Springer Protocols

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