Abstract
Proteasomes are multicatalytic proteases present in the nucleus and cytosol of eukaryotic cells. The central catalytic core, the 20S proteasome, consists of four heptameric rings, the central two of which contain the catalytic β-sub- units, members of a new family of threonine (Thr)-proteases. The outer rings, made of α-subunits, bind the regulators that control the substrate specificity of the proteasome. The binding of a 19S regulator to each end of the 20S core creates the 26S proteasome, which degrades ubiquitinated substrates in an adenosine triphosphate-dependent manner (1,2).
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References
Baumeister, W., Walz, J., Zühl, F., and Seemüller, E. (1998) Proteasome: paradigm of a self-compartmentalizing protease. Cell 92, 367–380.
Hoffman, L., and Rechsteiner, M. (1996) Regulatory features of multicatalytic and 26S proteasomes. Curr. Top. Cell Regul. 34, 1–32.
Yewdell, J. W. and Bennink, J. R. (1992) Cell biology of antigen processing and presentation to Major Histocompatibility Complex class I molecule-restricted T lymphocytes. Adv. Immunol. 52, 1–123.
Goldberg, A. L. and Rock, K. L. (1992) Proteolysis, proteasomes and antigen processing. Nature 357, 375–379.
Nandi, D., Marusina, K., and Monaco, J. J. (1998) How do endogenous proteins become peptides and reach the endoplasmic reticulum. Curr. Top. Microbiol. Immunol. 232, 15–47.
Rock, K. L., Gramm, C., Rothstein, L., Clark, K., Stein, R., Dick, L., Hwang, D., and Goldberg, A. L. Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules. Cell 78, 761–771.
Bogyo, M., Gaczynska, M., and Ploegh, H. L. (1997) Proteasome inhibitors and antigen presentation. Biopolymers 43, 269–280.
Vinitsky, A., Michaud, C., Powers, J. C., and Orlowski, M. (1992) Inhibition of the chymotrypsin-like activity of the pituitary multicatalytic proteinase complex. Biochemistry 31, 9421–9428.
Löwe, J., Stock, D., Jap, B., Zwickl, P., Baumeister, W., and Huber, R. (1995) Crystal structure of the 20S proteasome from the Archaeon T. Acidophilum at 3.4 A resolution. Science 268, 533–539.
Fenteany, G., Standaert, R. F., Lane, W. S., Choi, S., Corey, E. J., and Schreiber, S. L. (1995) Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 268, 726–731.
Dick, L. R., Cruikshank, A. A., Grenier, L., Melandri, F. D., Nunes, S. L., and Stein, R. L. (1996) Mechanistic studies on the inactivation of the proteasome by lactacystin. A central role for clasto-lactacystin β-lactone. J. Biol. Chem. 271, 7273–7276.
Ostrowska, H., Wojcik, C., Omura, S., and Worowski, K. (1997) Lactacystin, a specific inhibitor of the proteasome, inhibits human platelet lysosomal chathepsin A-like enzyme. Biochem. Biophys. Res. Commun. 234, 729–732.
Bogyo, M., McMaster, J. S., Gaczynska, M., Tortorella, D., Goldberg, A. L., and Ploegh, H. L. (1997) Covalent modification of the active site threonine of proteasomal β subunits and the Escherichia coli homolog HslV by a new class of inhibitors. Proc. Natl. Acad. Sci. USA 94, 6629–6634.
McCormack, T., Baumeister, W., Grenier, L., Moomaw, C., Plamondon, L., Pramanik, B., et al. Active site inhibitors of Rhodococcus 20 S proteasome. Kinetics and mechanism. J. Biol. Chem. 272, 26,103–26,109.
Bachmair, A., Finley, D., and Varshavski, A. (1986) In vivo half-life of a protein is a function of its amino-terminal residue. Science 234, 179–186.
Varshavsky, A. (1996) N-end rule: functions, mysteries, uses. Proc. Natl. Acad. Sci. USA 93, 12,142–12,149.
Townsend, A., Bastin, J., Gould, K., Brownlee, G., Andrew, M., Coupar, B., Boyle, D., Chan, S., and Smith, G. (1988) Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen. J. Exp. Med. 168, 1211–1224.
Porgador, A., Yewdell, J. W., Deng, Y., Bennink, J. R., and Germain, R. N. (1997) Localization, quantitation, and in situ detection of specific peptide-MHC class I complexes using a monoclonal antibody. Immunity 6, 715–726.
Andersen, P. S., Stryhn, A., Hansen, B. E., Fugger, L., Engberg, J., and Buus, S. (1996) Recombinant antibody with the antigen-specific, major histo-compatibility complex-restricted specificity. Proc. Natl. Acad. Sci. USA 93, 1820–1824.
Mimnaugh, E. G., Chen, H. Y., Davie, J. R., Celis, J. E., and Neckers, L. (1997) Rapid deubiquitination of nucleosomal histones in human tumor cells caused by proteasome inhibitors and stress response inducers: effects on replication, transcription, translation, and cellular stress. Biochemistry 36, 14,418–14,429.
Zhou, M., Wu, X., and Ginsberg, H. N. (1996) Evidence that a rapidly turning over protein, normally degraded by proteasomes, regulates hsp72 gene transcription in HepG2 cells. J. Biol. Chem. 271, 24,769–24,775.
Bush, K. T., Goldberg, A. L., and Nigam, S. K. (1997) Proteasome inhibition leads to a heat-shock response, induction of endoplasmic reticulum chaperones, and thermotolerance. j. Biol. Chem. 272, 9086–9092.
Kawazoe, Y., Nakai, A., Tanabe, M., and Nagata, K. (1998) Proteasome inhibition leads to the activation of all members of the heat-shock-factor family. Eur. J. Biochem. 255, 356–362.
Hershko, A. and Ciechanover, A. (1998) The ubiquitin system. Ann. Rev. Biochem. 67, 425–479.
Grimm, L. M. and Osborne, B. A. (1999) Apoptosis and the proteasome. Results Probl. Cell Differ. 23, 209–228.
Meriin, A. B., Gabai, V. L., Yaglom, J., Shifrin, V. I., and Sherman, M. Y.(1998) Proteasome inhibitors activate stress kinases and induce Hsp72. Diverse effects on apoptosis. j. Biol. Chem. 273, 6373–6379.
Yellen-Shaw, A. J. and Eisenlohr, L.C. (1997) Regulation of class I-restricted epitope processing by local or distal flanking sequence. j. Immunol. 158, 1727–1733.
Vinitsky, A., Antón, L. C., Snyder, H. L., Orlowski, M., Bennink, J. R., and Yewdell, J. W. Generation of MHC class I-associated peptides is only partially inhibited by proteasome inhibitors. Involvement of nonproteasomal proteases in antigen processing? j. Immunol. 159, 554–564.
Cerundolo, V., Benham, A., Braud, V., Mukherjee, S., Gould, K., Macino, B., Neefjes, J., and Townsend, A. (1997) Proteasome-specific inhibitor lactacystin blocks presentation of cytotoxic T lymphocyte epitopes in human and murine cells. Eur. J. Immunol. 27, 336–341.
Antón, L. C., Snyder, H. L., Bennink, J. R., Vinitsky, A., Orlowski, M., Porgador, A., and Yewdell, J. W. (1998) Dissociation of proteasomal degradation of biosynthesized viral proteins from generation of MHC class I-associated antigenic peptides. j. Immunol. 160, 4859–4868.
Benham, A., Grommé, V, and Neefjes, J. (1998) Allelic differences in the relationship between proteasome activity and MHC class I peptide loading. j. Immunol. 161, 83–89.
Luckey, C. J., King, G. M., Marto, J. A., Venketeswaran, S., Maier, B. F., Crotzer, V. L., et al. (1998) Proteasomes can either generate or destroy MHC class I epitopes: evidence for nonproteasomal epitope generation in the cytosol. j. Immunol. 161, 112–121.
Glas, R., Bogyo, M., McMaster, J. S., Gaczynska, M., and Ploegh, H. L. (1998) A proteolytic system that compensates for loss of proteasome function. Nature 392, 618–622.
Geier, E., Pfeifer, G., Wilm, M., Lucchiari-Hartz, M., Baumeister, W., Eichmann, K., and Niedermann, G. (1999) Giant protease with potential to substitute for some functions of the proteasome. Science 283, 978–981.
Williams, D. B., Swiedler, S. J., and Hart, G. W. (1985) Intracellular transport of membrane glycoproteins: two closely related histocompatibility antigens differ in their rates of transit to the cell surface. j. Cell Biol. 101, 725–734.
Moore, M. W., Carbone, F. R., and Bevan, M. J. (1988) Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54, 777–785.
Dick, L. R., Cruikshank, A. A., Destree, A. T., Grenier, L., McCormack, T. A., Melandri, F. D., et al. (1997) Mechanistic studies on the inactivation of the proteasome by lactacystin in cultured cells. j. Biol. Chem. 272, 182–188.
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Antón, L.C., Bennink, J.R., Yewdell, J.W. (2000). Use of Proteasome Inhibitors to Examine Processing of Antigens for Major Histocompatibility Complex Class I Presentation. In: Solheim, J.C. (eds) Antigen Processing and Presentation Protocols. Methods in Molecular Biology, vol 156. Humana Press. https://doi.org/10.1385/1-59259-062-4:17
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DOI: https://doi.org/10.1385/1-59259-062-4:17
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