Assay of Malate Dehydrogenase

A Substrate for the E. coli Chaperonins GroEL and GroES
  • Manajit Hayer-Hartl
Part of the Methods in Molecular Biology™ book series (MIMB, volume 140)


Malate dehydrogenase (MDH) is found in all eukaryotic cells as two isozymes:mitochondrial (mMDH) and cytoplasmic (soluble, sMDH) (1). In constrast, prokaryotes contain only a single form of MDH. The crystal structures of MDH from Escherichia coli (2 3), porcine cytoplasm (4), porcine mitochondria (5 6), and Thermus flavus (7) have been solved and are essentially identical. Refer to ref. (8) for a comprehensive review on MDH.


Creatine Kinase Pyruvate Kinase Assay Mixture Malate Dehydrogenase Single Form 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Manajit Hayer-Hartl
    • 1
  1. 1.Department of Cellular BiochemistryMax Planck Institute of BiochemistyMartinsriedGermany

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