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Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins

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Protein Kinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 124))

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Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is fast becoming the marker of choice for protein localization and gene expression studies. The rapid rise in popularity stems from several advantages over traditional markers such as β-galactosidase and alkaline phosphatase, which typically require the addition of exogenous substrates for detection. The unique intrinsic spectral properties of GFP allow it to be directly visualized in fixed or live cells by fluorescence microscopy without the addition of cofactors or exogenous substrates (1–8).

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Authors
  1. Ralph A. Zirngibl
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  2. Peter Greer
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    © 2000 Humana Press Inc., Totowa, NJ

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    Zirngibl, R.A., Greer, P. (2000). Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:195

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    • DOI: https://doi.org/10.1385/1-59259-059-4:195

    • Publisher Name: Humana Press

    • Print ISBN: 978-0-89603-700-7

    • Online ISBN: 978-1-59259-059-9

    • eBook Packages: Springer Protocols

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