Abstract
The activation of T cells results in both tyrosine and serine/threonine phos-phorylations of a number of substrates within minutes of T-cell receptor (TCR) ligation (1,2). The phosphorylation of the immunoreceptor tyrosine-based activation motifs (ITAMs) on the (ξ-chain within the TCR complex act as binding sites for the N-terminal SH2 domains of ZAP-70 (3). This relocation brings ZAP-70 proximal to the CD4-associated p56lck kinase, which activates the kinase activity of ZAP-70 by phosphorylation at Y493 (4). The activated ZAP-70 is then able to transduce a signal via an as yet uncharacterized cascade, which may involve SLP-76 (5), vav (6) PLC-Γ( (7) p120/130 (8) and LAT (9). The major role of ZAP-70 in T-cell signal transduction has been highlighted in ZAP-70 deficient patients who suffer severe combined immunodeficiency disease (SCID) like symptoms (10).
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© 2000 Humana Press Inc., Totowa, NJ
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Purton, T., Wilkinson, S., Murray, E.J. (2000). Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:183
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DOI: https://doi.org/10.1385/1-59259-059-4:183
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