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Capillary Electrophoresis of Nucleic Acids pp 353–370Cite as

Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 162))

Abstract

Short segments of DNA, which have been synthesized chemically, are commonly known as synthetic oligonucleotides (ODNs). The length of the ODNs typically utilized in the lab varies but is on average between five and forty nucleotides. Chemical modifications may be selectively placed at various locations within the molecule including the backbone, the heterocyclic base, or the sugar moiety (1,2). For the work discussed in this chapter, chemical alterations were placed in three locations: the backbone, the 3’-bridging position between the backbone and the sugar moiety, and at the 5’ end.

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Author information

Authors and Affiliations

  1. Lynx Therapeutics, Hayward, CA

    Lawrence A. DeDionisio

Authors
  1. Lawrence A. DeDionisio
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Editor information

Editors and Affiliations

  1. Australian Genome Research Facility, University of Queensland, Brisbane

    Keith R. Mitchelson

  2. Walter & Eliza Hall Institute, Parkville, Australia

    Keith R. Mitchelson

  3. Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

    Jing Cheng

  4. Aviva Biosciences Corporation, San Diego, CA

    Jing Cheng

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© 2001 Humana Press Inc.

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DeDionisio, L.A. (2001). Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:353

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  • DOI: https://doi.org/10.1385/1-59259-055-1:353

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-779-3

  • Online ISBN: 978-1-59259-055-1

  • eBook Packages: Springer Protocols

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