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Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels

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Capillary Electrophoresis of Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 162))

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Abstract

Capillary electrophoresis (CE) in all its various modes is routinely used in the area of life science for the analysis of wide spectra of species ranging from small inorganic/ organic molecules to multi-subunit proteins and mega-size DNA (1). Capillary gel electrophoresis (CGE) is the counterpart of gel electrophoresis in the slab-gel format. Similar molecules are analyzed with CGE as with slab gels, like SDS-protein complexes and single-stranded/duplex DNA. Since these analytes have similar mass-overcharge ratio and therefore similar mobilities (for ≥10 bases in length, depending on pH and base composition) they can not be separated in a carrier-free system like capillary free-zone electrophoresis (2), although some exceptions are known (3,4). A size-sieving medium is therefore necessary to impart hydrodynamic friction on the migrating molecules for their separation. The constraint of the capillary format also acts to prevent convection, whereas in the slab-gel format the sieving matrix plays this role.

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Author information

Authors and Affiliations

  1. Molecular Sciences, AstraZeneca R&D Lund, Lund, Sweden

    Anders K. Palm

Authors
  1. Anders K. Palm
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Editor information

Editors and Affiliations

  1. Australian Genome Research Facility, University of Queensland, Brisbane

    Keith R. Mitchelson

  2. Walter & Eliza Hall Institute, Parkville, Australia

    Keith R. Mitchelson

  3. Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

    Jing Cheng

  4. Aviva Biosciences Corporation, San Diego, CA

    Jing Cheng

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© 2001 Humana Press Inc.

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Palm, A.K. (2001). Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:279

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  • DOI: https://doi.org/10.1385/1-59259-055-1:279

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-779-3

  • Online ISBN: 978-1-59259-055-1

  • eBook Packages: Springer Protocols

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