Abstract
Capillary electrophoresis (CE) has been recognized as a precision tool, which is an excellent analytical technique with high sensitivity, high resolution, and has a robust ability for quantitation of the amount of analyte. It also has proven to be a versatile tool for the separation of an enormous range of molecular sizes, from small molecules such as inorganic cations, anions, and drugs, through to large molecules such as peptides, proteins, carbohydrates, polysaccharides, DNA fragments, and even whole chromosomes.
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References
Vincent U., Patra G., Therasse J., and Gareil P. (1996) Quantitation of polymerase chain reaction-amplified DNA fragments by capillary electrophoresis and laser-induced fluorescence detection. Electrophoresis 17, 512–517.
Lu W., Han D. S., and Andrieu J. M. (1994) Multitarget PCR analysis by capillary electrophoresis and laser-induced fluorescence detection. Nature 368, 269–271.
Kim Y. and Morris M. D. (1994) Separation of nucleic acids by capillary electrophoresis in cellulose solutions with mono-and bis-intercalating dyes. Anal. Chem. 66, 1168–1174.
Chang H. and Yeung E. S. (1995) Poly(ethyelene oxide) for high-resolution and highspeed separation of DNA by capillary electrophoresis. J. Chromatogr. B 669, 111–123.
Zhang N. and Yeung E. S. (1996) Genetic typing by capillary electrophoresis with the allelic ladders as an absolute standard. Anal. Chem. 68, 2927–2931.
Michael A. M., Devamey J. M., Davis P. A., Smith J. K., and Girard J. E. (1998) Spectral measurement of intercalated PCR-amplified short tandem repeat alleles. Anal. Chem. 7,4514–4519.
Kheterpal I. and Mathies R. A. (1999) Capillary array electrophoresis DNA sequencing. Anal. Chem. 71, 31A–37A.
Smith L. M. (1991) High-speed DNA sequencing by capillary gel-electrophoresis. Nature 349,812–813.
Salas-Solano O., Carillho E., Kotler L., Miller A. W., Goetzinger W., Sosic Z., and Karger B. L. (1998) Routine DNA sequencing of 1000 bases in less than hour by capillary electrophoresis with replaceable linear polyacrylamide solutions. Anal. Chem. 70, 3996–4003.
Kamahori M. and Kambara H. (2001) Capillary array electrophoresis analyzer, in Capillary Electrophoresis of Nucleic Acids, Vol. 2 (Mitchelson K. R. and Cheng J., eds.), Humana Press, Totowa, NJ, pp. 271–287.
Ruiz-Martinez M. C., Berka J., Blelnkii A., Foret F., Miller A. W., and Karger B. L. (1993) DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection. Anal. Chem. 65, 2851–2858.
Crehan W. A. M., Rasmussen H. T., and Northrop D. M. (1992) Size-selective capillary electrophoresis (SSCE) separation of DNA fragments. J. Liq. Chromatogr. 15,1063–1080.
Grossman P. D. and Soane D. S. (1991) Capillary eletrophoresis of DNA in entangled polymer solutions. J. Chromatogr. 559, 257–266.
Zewert T. and Harrington M. (1992) Polyhydroxy and polyethyleneglycol (meth) acrylate polymers-physical properties and general studies for their use as electrophoresis matrices. Electrophoresis 13, 817–824.
Kleemib M. H., Gilges M., and Schomburg G. (1993) Capillary electrophoresis of DNA restriction fragments with solutions of entangled polymers. Electrophoresis 14, 515–522.
Zhu M., Hansen D. L., Burd S., and Gannon F. (1989) Factors affecting free zone electrophoresis and isoelectric focusing in capillary electrophoresis. J. Chromatogr. 480,311–319.
Menchen S., Johnson B., Winnik M. A., and Xu B. (1996) Flowable network as DNA sequencing media in capillary columns. Electrophoresis 17, 1451–1459.
Sassi A. P., Barron A., Alonso-Amigo M. G., Hion D. Y., Yu J. S., and Soane D. S. (1996) Electrophoresis of DNA in novel thermoreversible matrices. Electrophoresis 17, 1460–1469.
Balijepalli S. and Schultz J. M. (1996) Phase behavior and morphology of poly(ethylene oxide) blends. Macromolecules 29, 6601–6611.
Chen J. H., Cheng S. Z. D., Wu S. S., Lotz B., and Wittmann J. C. (1995) Polymer decoration study in chain folding behavior of solution-grown poly(ehtylene oxide) crystals. J. Polym. Sci. B. Polym. Phys. 33, 1851–1855.
Cheng S. Z. D. and Chen J. H. (1991) Nonintegral and integral folding crystal-growth in low-molecular mass poly(ethylene oxide) fractions. 3. Linear crystal-growth rates and crystal morphology. J. Polym. Sci. B. Polym. Phys. 29, 311–327.
Bieze T. W. N., van der Maarel J. R. C., Eisenbach C. D., and Leyte J. C. (1994) Polymer dynamics in aqueous poly(ethylene oxide) solutions-an NMR study. Macromolecules 27, 1355–1366.
Merdas A., Gindre M., Ober R., Nicot C., Urbach W., and Waks M. (1996) Nonionic surfactant reverse micelles of C(12)E(4) in dodecane: temperature dependence of size and shape. J. Phys. Chem. 100,15,180–15,186.
Briscoe B., Luckham P., and Zhu S. (1996) Rheological study of poly(ethylene oxide) in aqueous salt solutions at high temperature and pressure. Macromolecules 29, 6208–6211.
Kim Y. and Yeung E. S. (1997) Separation of DNA sequencing fragments up to 1000 bases by using poly(ethylene oxide)-filled capillary electrophoresis. J. Chromatogr. A 781, 315–325.
Kim Y. and Morris M. D. (1994) Pulsed field capillary electrophoresis of multi-kilobase length nucleic acids in dilute methyl cellulose solutions. Anal. Chem. 66, 3081–3085.
Heller C., Magnúsdóttir S., and Viovy J.-L. (2001) Robust field inversion capillary electrophoretic separation of long DNA fragments, in Capillary Electrophoresis of Nucleic Acids, Vol. 1 (Mitchelson K. R. and Cheng J., eds.), Humana Press, Totowa, NJ, pp. 293–305.
Morris M. D., Schwinefus J. J., and de Carmejane O. (2001) Pulsed-field capillary electrophoresis separation of large DNA fragments, in Capillary Electrophoresis of Nucleic Acids, Vol. 1 (Mitchelson K. R. and Cheng J., eds.), Humana Press, Totowa, NJ, pp. 307–321.
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Kim, Y., Yeung, E.S. (2001). Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:215
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DOI: https://doi.org/10.1385/1-59259-055-1:215
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Print ISBN: 978-0-89603-779-3
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