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Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis

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Capillary Electrophoresis of Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 162))

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Abstract

In this chapter, we examine important parameters that affect the performance of replaceable polymers currently used for DNA sequencing by capillary electrophoresis (DNASCE). Background on physical models used to describe polymer solutions and DNA migration in semi-dilute polymer solution will be given to provide a framework for interpretation of experimental data and provide estimates of optimized polymer and electrophoresis parameters. Discussion of parameters affecting DNASCE resolution will be followed by comparison of experimental conditions for four linear polymers currently used in DNASCE (1–10). Recent developments in performance optimization and inner-wall coatings will also be discussed.

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Author information

Authors and Affiliations

  1. Science & Technology, Corning Incorporated, Corning, NY

    Mark A. Quesada

  2. Applied Biosystems Corporation, Foster City, CA

    Steve Menchen

Authors
  1. Mark A. Quesada
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  2. Steve Menchen
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Editor information

Editors and Affiliations

  1. Australian Genome Research Facility, University of Queensland, Brisbane

    Keith R. Mitchelson

  2. Walter & Eliza Hall Institute, Parkville, Australia

    Keith R. Mitchelson

  3. Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

    Jing Cheng

  4. Aviva Biosciences Corporation, San Diego, CA

    Jing Cheng

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Quesada, M.A., Menchen, S. (2001). Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology, vol 162. Humana Press. https://doi.org/10.1385/1-59259-055-1:139

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  • DOI: https://doi.org/10.1385/1-59259-055-1:139

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-779-3

  • Online ISBN: 978-1-59259-055-1

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