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Transforming Growth Factor-Beta Protocols pp 149–167Cite as

Analysis of TGFβ-Inducible Apoptosis

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 142))

Abstract

Transforming growth factor-β (TGF-β) is an important regulator of cellular growth and immune homeostasis (1–3). TGFβ is a homodimeric 25-kDa protein which activates cellular signaling through the recruitment and transphos-phorylation of specific heterodimeric cell-surface receptors (4). Although activation of TGF-β receptors initiates serine/threonine kinase activity, the subsequent signaling mechanisms involved in a wide array of diverse functional consequences is currently under investigation. TGF-β has been postulated to play important roles in tissue fibrosis and the regulation of the extracellular matrix (5), growth arrest of epithelial cells (6), regulation of the immune response, and induction of apoptotic cell death (7–11). The essential nature of this cytokine is exemplified by the lethal and nonoverlapping phenotypes of the three highly homologous mammalian TGF-β isoforms, each of which exhibit cell-specific expression (3,12). TGF-β1 deficient mice exhibit extensive lymphocytic hyperproliferation and the production of autoimmune antibodies found in several human diseases (3,13–16).

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Author information

Authors and Affiliations

  1. Department of Cell Biology,Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH

    Thomas L. Brown, Supriya Patil & Philip H. Howe

Authors
  1. Thomas L. Brown
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  2. Supriya Patil
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  3. Philip H. Howe
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Editors and Affiliations

  1. The Cleveland Clinic Foundation, Cleveland, OH

    Philip H. Howe

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© 2000 Humana Press Inc.

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Brown, T.L., Patil, S., Howe, P.H. (2000). Analysis of TGFβ-Inducible Apoptosis. In: Howe, P.H. (eds) Transforming Growth Factor-Beta Protocols. Methods in Molecular Biology™, vol 142. Humana Press. https://doi.org/10.1385/1-59259-053-5:149

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  • DOI: https://doi.org/10.1385/1-59259-053-5:149

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-646-8

  • Online ISBN: 978-1-59259-053-7

  • eBook Packages: Springer Protocols

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