Abstract
Transforming growth factors (TGFs) were initially isolated from the conditioned medium of transformed cell lines through their ability to stimulate anchorage-dependent cells to form colonies in soft agar (1,2). The ability to proliferate in an anchorage-independent manner is still one of the best in vitro correlates with tumorigenicity. Subsequent studies demonstrated that the growth-promoting activity in the conditioned medium consisted of two unique peptides, TGF-α and TGF-β (3–5). Depending on the indicator cell line used, soft-agar colony growth could occur when TGF-α and TGF-β (i.e., NRK cells) or TGF-β alone (i.e., AKR-2B cells) were added to the serum-containing medium (6,7). This review will focus on TGF-β and cellular systems capable of responding in vitro to its growth modulatory activity independent of additional factors.
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Edens, M., Leof, E.B. (2000). In Vitro Assays for Measuring TGFβ Growth Stimulation and Inhibition. In: Howe, P.H. (eds) Transforming Growth Factor-Beta Protocols. Methods in Molecular Biology™, vol 142. Humana Press. https://doi.org/10.1385/1-59259-053-5:1
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DOI: https://doi.org/10.1385/1-59259-053-5:1
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