Detection of TGFβ in Body Fluids and Tissues

  • Andrew H. Limper
Part of the Methods in Molecular Biology™ book series (MIMB, volume 142)

Abstract

Transforming growth factor-β1 (TGF-(β1) is a 25-kDa homodimeric protein secreted by numerous cells including platelets, monocytes, macrophages, epithelial cells, and fibroblasts (1, 2, 3). Active TGF-(β1 exerts multiple and divergent effects on mesenchymal and epithelial cell cycle regulation (1). In addition, TGF-(β1 influences the expression of multiple genes, which act in concert to enhance the synthesis of extracellular matrix components with resulting fibrosis (1,2). In vitro studies have demonstrated that TGF-(β1 regulates the genetic expression of extracellular matrix components, including fibronectin (FN), the α5β1 fibronectin receptor (FNR), collagen, and proteoglycans (4, 5, 6, 7).

Keywords

Formalin Magnesium EDTA Petroleum Chloroform 

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Andrew H. Limper
    • 1
  1. 1.Department of MedicineBiochemistry and Molecular Biology, Mayo ClinicRochester

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