Abstract
Chromatophores are pigmented cells found in a variety of animals. In lower vertebrates, i.e., fish and amphibia, chromatophores are present in the dermis, and in response to various stimuli, the pigment in these cells is transported to or from the cell center, conferring upon the cell a light or dark appearance, respectively. Changes in the distribution of pigment permit the animal to display variations in coloration which are used for territorial or sexual display and for camouflage. While color change is an important component of animal behavior, these cells have been exploited for cell biological studies, providing an excellent model system for examination of mechanisms generating and regulating organelle transport (1).
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References
Bagnara J. T. and Hadley M. E. (1973) Chromatophores and color change. The Comparative Physiology of Animal Pigmentation. Prentice-Hall, Englewood Cliffs.
Murphy D. B. and Tilney L. G. (1974) The role of microtubules in the movement of pigment granules in teleost melanophores. J. Cell Biol. 61, 757–779.
Thaler C. D. and Haimo L. T. (1992) Control of organelle transport in melanophores: regulation of Ca2+ and cAMP levels. CellMotil. Cytoskel. 22, 175–184.
Schliwa M., Weber K., and Porter K. R. (1981) Localization and organization of actin in melanophores. J. Cell Biol. 89, 267–275.
Luby K. J. and Porter K. R. (1980) The control of pigment migration in isolated erythrophores of Holocentrus ascensionis (Osbeck). I. Energy requirements. Cell 21, 13–23.
Daniolos A., Lerner A. B., and Lerner M. R. (1990) Action of light on frog pigment cells in culture. Pigment Cell Research 3, 38–43.
Clark T. G. and Rosenbaum J. L. (1982) Pigment particle translocation in detergent permeabilized melanophores of Fundulus heteroclitus. Proc. Natl. Acad. Sci. USA 79, 4655–4659.
Rozdzial M. M. and Haimo L. T. (1986) Reactivated melanophore motility: Differential regulation and nucleotide requirements of bidirectional pigment granule transport. J. Cell Biol. 103, 2755–2764.
Grumstrom N., Karlsson J. O. G., and Andersson R. G. G. (1985) The control of granule movement in fish melanophores. Acta Physiol. Scand. 125, 415–422.
Lynch T. J., Wu B., Taylor J. D., and Tchen T. T. (1986) Regulation of pigment organelle translocation. II. Participation of a cAMP-dependent protein kinase. J. Biol. Chem. 261, 4212–4216.
Rodionov V. I. and Borisy G. G. (1997) Cell-centring activity of cytoplasm. Nature 386, 170–173.
Graminski G. F., Jayawickreme C. K., Potenza M. M., and Lerner M. R. (1993) Pigment dispersion in frog melanophores can be induced by a phorbol ester or stimulation of a recombinant receptor that activates phospholipase C. J. Biol. Chem. 268, 5957–5964.
Reinlein A. R., Tint I. S., Peunova N. I., Enikolopov G. N., and Gelfand V. I. (1998) Regulation of organelle movement in melanophores by protein kinase A (PKA), protein kinase C (PKC), and protein phosphatase 2A (PP2A). J. Cell Biol. 142, 803–813.
Tuma M. C., Zill A., Le Bot N., Vernos I., and Gelfand V. (1998) Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores. J. Cell Biol. 143, 1547–1558.
Rogers S. L., Tint I. S., Fanapour P. C., and Gelfand V. I. (1997) Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro. Proc. Natl. Acad. Sci. USA 94, 3720–3725.
Nilsson H. and Wallin M. (1998) Microtubule aster formation by dynein-depen-dent organelle transport. Cell Motil. Cytoskel. 41, 254–263.
Rodionov V. I., Gyoeva F. K., and Gelfand V. I. (1991) Kinesin is responsible for centrifugal movement of pigment granules in melanophores. Proc. Natl. Acad. Sci. USA 88, 4956–4960.
Nilsson H. and Wallin M. (1997) Evidence for several roles of dynein in pigment transport in melanophores. Cell Motil. Cytoskel. 38, 397–409.
Malawista S. E. (1971) Cytochalasin B reversibly inhibits melanin granule movement in melanocytes. Nature (Lond) 234, 354–355.
Rogers S. L. and Gelfand V. I. (1998) Myosin cooperates with microtubule motors during organelle transport in melanophores. Curr. Biol. 8, 161–164.
Rodionov V. I., Hope A. J., Svitkina T. M., and Borisy G. G. (1998) Functional coordination of microtubule-based and actin-based motility in melanophores. Curr. Biol. 8, 165–168.
Rozdzial M. M. and Haimo L. T. (1986) Bidirectional pigment granule movements of melanophores are regulated by protein phosphorylation and dephosphor-ylation. Cell 47, 1061–1070.
Sammack P. J., Adams S. R., Harootunian A. T., Schliwa M., and Tsien R. Y. (1992) Intracellular cyclic AMP, not calcium, determine the direction of vesicle movement in melanophores: direct measurement by fluorescence ratio imaging. J. Cell Biol. 117, 57–72.
Sugden D. and Rowe S. J. (1992) Protein kinase C activation antagonizes mela-tonin-induced pigment aggregation in Xenopus laevis melanophores. J. Cell Biol. 119, 1515–1521.
Thaler C. D. and Haimo L. T. (1990) Regulation of organelle transport in melanophores by calcineurin. J. Cell Biol. 111, 1939–1948.
Haimo L. T. (1998) Reactivation of vesicle transport in lysed teleost melanophores. In: Methods in Enzymology, Vol. 298, (Vallee R. B., ed.), Academic Press, San Diego, pp. 389–399.
Rogers S. L., Tint I. S., and Gelfand V. I. (1998) In vitro motility assay for melanophore pigment organelles. In: Methods in Enzymology, Vol. 298, (Vallee R. B., ed.), Academic Press, San Diego, pp. 361–372.
Gyoeva F. K., Leonova E. V., Rodionov V. I., and Gelfand V. I. (1987) Vimentin intermediate filaments in fish melanophores. J. Cell Sci. 88, 649–655.
Rodionov V. I., Lim S., Gelfand V. I., and Borisy G. G. (1994) Microtubule dynamics in fish melanophores. J. Cell Biol. 126, 1455–1464.
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Telzerand, B.R., Haimo, L.T. (2001). Chromatophores as Tools for the Study of Organelle Transport. In: Gavin, R.H. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology™, vol 161. Humana Press. https://doi.org/10.1385/1-59259-051-9:201
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DOI: https://doi.org/10.1385/1-59259-051-9:201
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