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Cytoskeleton Methods and Protocols pp 109–120Cite as

Jasplakinolide

An Actin-Specific Reagent that Promotes Actin Polymerization

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 161))

Abstract

The actin system is involved in different cellular processes such as exo- and endocytosis, organelle motions, maintenance of organelle distribution, motility, cell division, and cytoplasmic streaming in plant cells. Several substances are known to influence these processes by altering intracellular actin organization and have been used extensively for cell biological research. Their mechanisms of action are mostly destructive on actin filaments (F-actin), either by inhibition of F-actin elongation as in the case of cytochalasins (1) or by interaction with actin monomers (G-actin) in order to avoid actin polymerization as described for latrunculins (2). In contrast, F-actin polymerizing and stabilizing capacities were found for jasplakinolide (3–6). Moreover jasplakinolide has the valuable advantage of being membrane permeable (7).

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Author information

Authors and Affiliations

  1. Institute for Plant Physiology, University of Salzburg, Salzburg, Austria

    Andreas Holzinger

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  1. Andreas Holzinger
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Editor information

Editors and Affiliations

  1. Department of Biology, Brooklyn College, The City University of New York, NY

    Ray H. Gavin

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© 2001 Humana Press Inc.

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Holzinger, A. (2001). Jasplakinolide. In: Gavin, R.H. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology™, vol 161. Humana Press. https://doi.org/10.1385/1-59259-051-9:109

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  • DOI: https://doi.org/10.1385/1-59259-051-9:109

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-771-7

  • Online ISBN: 978-1-59259-051-3

  • eBook Packages: Springer Protocols

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