Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function

  • George M. Langford
Part of the Methods in Molecular Biology™ book series (MIMB, volume 161)

Abstract

The cytoskeleton is a dynamic network of filaments in the cytoplasm of cells and functions as the roadways for vesicular transport. Of the three types of cytoskeletal filaments, both microtubules and actin filaments have been shown to support vesicle transport. The transport of vesicles is mediated by molecular motors and members of all three super-families of molecular motors-myosin, kinesin and cytoplasmic dynein-have been shown to function as vesicle motors (1, 2, 3, 4). The specific types of vesicles transported by some of the molecular motors have been identified (4, 5, 6).

Keywords

Quartz Dust Depression Mercury Recombination 

References

  1. 1.
    Langford G. M. and Molyneaux B. J. (1998) Myosin V in the brain: mutations lead to neurological defects, Brain Res. Rev. 28, 1–8.PubMedCrossRefGoogle Scholar
  2. 2.
    Mermall V., Post P. L., and Mooseker M. S. (1998) Unconventional myo-sins in cell movement, membrane traffic, and signal transduction. Science 279, 527–533.PubMedCrossRefGoogle Scholar
  3. 3.
    Karki S. and Holzbaur E. L. (1999) Cytoplasmic dynein and dynactin in cell division and intracellular transport. Curr. Opin. Cell. Biol. 11, 45–53.PubMedCrossRefGoogle Scholar
  4. 4.
    Hirokawa N. (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279, 519–526.PubMedCrossRefGoogle Scholar
  5. 5.
    DePina A. S. and Langford G. M. (1999) Vesicle Transport: the role of actin filaments and myosin motors. Microsc. Res. Tech. 47, 93–106.PubMedCrossRefGoogle Scholar
  6. 6.
    Wu X., Bowers B., Rao K., Wei Q., and Hammer J. A., 3rd. (1998) Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo. J. Cell. Biol. 143, 1899–1918.PubMedCrossRefGoogle Scholar
  7. 7.
    Allen R. D., Weiss D. G., Hayden J. H., Brown D. T., Fujiwake H., and Simpson M. (1985) Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: Evidence for an active role of micro-tubules in cytoplasmic transport. J. Cell. Biol. 100, 1736–1752.PubMedCrossRefGoogle Scholar
  8. 8.
    Vale R. D., Schnapp B. J., Reese T. S., and Sheetz M. P. (1985) Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. Cell 40, 559–569.PubMedCrossRefGoogle Scholar
  9. 9.
    Kuznetsov S. A., Langford G. M., and Weiss D. G. (1992) Actin-dependent organelle movement in squid axoplasm. Nature 356, 725–727.CrossRefGoogle Scholar
  10. 10.
    Allen R. D., Allen N. S., and Travis J. L. (1981) Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: A new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris. Cell Motil. 1, 291–302.PubMedCrossRefGoogle Scholar
  11. 11.
    Allen R. D., Travis J. L., Allen N. S., and Yilmaz H. (1981b) Video-enhanced contrast polarization (AVEC-POL) microscopy: A new method applied to the detection of birefringence in the motil reiculopodial network of Allogromia laticollaris. Cell Motil. 1, 275–288.PubMedCrossRefGoogle Scholar
  12. 12.
    Inoué S. (1981) Video image processing greatly enhances contrast, quality and speed in polarization-based microscopy. J. Cell. Biol. 89, 346–356.PubMedCrossRefGoogle Scholar
  13. 13.
    Inoué S. (1989) Imaging of unresolved objects, superresolution and precision of distance measurement with video microscopy. In “Methods in Cell Biology” (Eds. D. L. Taylor and Y.-L. Wang) Vol. 30, Pp. 85–112. Academic Press, New York.Google Scholar
  14. 14.
    Inoué S. (1986) Video Microscopy, 1st edition, Plenum Press, New York.Google Scholar
  15. 15.
    Inoué S. and Spring K. (1997) Video Microscopy, 2nd edition, Plenum Press, New York.Google Scholar
  16. 16.
    Weiss D. G. (1998) Video-enhanced contrast microscopy. In “Cell Biology: A Laboratory Handbook” Vol. 3, 2nd Edition, Pp. 99–108, Academic Press, New York.Google Scholar
  17. 17.
    Weiss D. G. and Maile W. (1993) Principles, practices and applications of video-enhanced contrast microscopy. In ldElectronic Light Microscopy” (Ed. D. Shotton), Pp. 105–140. Wiley-Liss, New York.Google Scholar
  18. 18.
    Weiss D. G., Maile W., and Wick R. A. (1989) Video microscopy. In “Light Microscopy in Biology. A Practical Approach” (Ed. A. J. Lacey), Pp. 221–278. IRL Press, Oxford.Google Scholar
  19. 19.
    Weiss D. G., Meyer M., and Langford G. M. (1990) Studying axoplasmic transport by video microscopy and using the squid giant axon as a model system. In “Squid as Experimental Animal” (Eds. D. L. Gilbert, W. J. Adelman, Jr., and J. M. Arnold), Pp. 303–321. Plenum Press, New York.Google Scholar
  20. 20.
    Bennett H. S. (1950) Methods applicable to the study of both fresh and fixed materials: The microscopical investigation of biological materials with polarized light. In “Handbook of Microscopical Technique” (Ed. C.E. McClung) Pp. 591–677, Harper and Row, New York.Google Scholar
  21. 21.
    Salmon E. D. and Tran P. (1998) High-resolution video-enhanced differential interference contrast (VE-DIC) light microscopy. In “Video Microscopy” Eds. G. Sluder and D. E. Wolf, Methods Cell. Biol. 56, 153–184, Academic Press, New York.Google Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • George M. Langford
    • 1
  1. 1.Department of Biological SciencesDartmouth CollegeHanover

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