Abstract
Although in recent years the molecular structure and function of calpains have been extensively investigated, some aspects of the roles and properties of these proteinases remain obscure, particularly their precise function in the cell, and the mechanisms inducing and promoting their activation.
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References
Davies E. V. and Hallett M. B. (1998) High micromolar Ca2+ beneath the plasma membrane in stimulated neutrophils. Biochem. Biophys. Res. Commun. 248, 679–683.
Mellgren R. L. and Lu Q. L. (1994) Selective nuclear transport of mu-calpain. Biochem. Biophys. Res. Commun . 204, 544–550.
Pontremoli S., Melloni E., Sparatore B., Salamino F., Michetti M., Sacco O., and Horecker B. L. (1985) Binding to erythrocyte membrane is the physiological mechanism for activation of Ca2+-dependent neutral proteinase. Biochem. Biophys. Res. Commun. 128, 331–338.
Croall D. E. and De Martino G. N. (1991) Calcium-activated neutral protease (calpain) system: Structure, function, and regulation. Physiol. Rev. 71, 813–847.
Pontremoli S., Melloni E., Michetti M., Salamino F., Sparatore B., and Horecker B. L. (1988) An endogenous activator of the Ca2+-dependent proteinase of human neutrophils that increases its affinity for Ca2+.Proc. Natl. Acad. Sci. USA 85, 1740–1743.
Pontremoli S., Viotti P. L., Michetti M., Sparatore B., Salamino F., and Melloni E. (1990) Identification of an endogenous activator of calpain in rat skeletal muscle. Biochem. Biophys. Res. Commun . 171, 569–574.
Melloni E., Michetti M., Salamino F., and Pontremoli S. (1998) Molecular and functional properties of a calpain activator protein specific for mu-isoforms. J. Biol. Chem. 273, 12827–12831.
Ceciliani F., Faotto L., Negri A., Colombo I., Berra B., Bartorelli A., and Ronchi S. (1996) The primary structure of UK114 tumour antigen. FEBS Lett. 393, 147–150.
Melloni E., Michetti M., Salamino F., Sparatore B., and Pontremoli S. (1998) Mechanism of action of a new component of the Ca2+-dependent proteolytic system in rat brain: The calpain activator. Biochem. Biophys. Res. Commun. 249, 583–588.
Udenfriend S., Stein S., Bohlen P., Dairman W., Leingruber W., and Weigele M. (1972) Fluorescamine: A reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178, 871–872.
Melloni E., Salamino F., Sparatore B., Michetti M., and Pontremoli S. (1984) Ca2+-dependent neutral proteinase from human erythrocytes: Activation by Ca2+ ions and substrate and regulation by the endogenous inhibitor. Biochem. Int. 8, 477–489.
Michetti M., Salamino F., Minafra R., Melloni E., and Pontremoli S. (1997) Calcium binding properties of human erythrocyte calpain. Biochem. J. 325, 721–726.
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Melloni, E., Michetti, M., Salamino, F., Minafra, R., Sparatore, B., Pontremoli, S. (2000). Isolation and Characterization of Calpain Activator Protein from Bovine Brain. In: Elce, J.S. (eds) Calpain Methods and Protocols. Methods in Molecular Biology™, vol 144. Humana Press. https://doi.org/10.1385/1-59259-050-0:99
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DOI: https://doi.org/10.1385/1-59259-050-0:99
Publisher Name: Humana Press
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