Abstract
Many of the early studies on the localization of calpain described a cytosolic distribution. However, it is becoming clear that calpain can play an essential role in regulating signal transduction across members of the integrin family of adhesion receptors and that in order to do this it is recruited to sites of ligand-occupied integrin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Meredith J. E., Jr., Winitz S., Lewis J. M., Hess S., Ren X.-D., Renshaw M. W., and Schwarts M. A. (1996) The regulation of growth and intracellular signaling by integrins. Endocr. Rev. 17, 207–220.
Lafrenie R. M. and Yamada K. M. (1996) Integrin-dependent signal transduction. J. Cell. Biochem. 61, 543–553.
Hynes R. O. (1992) Integrins: Versatility, modulation, and signaling cell adhesion. Cell 69, 11–25.
Price L. S., Leng J., Schwartz M. A., and Bokoch G. M. (1998) Activation of Rac and Cdc42 by integrin mediates cell spreading. Mol. Biol. Cell 9, 1863–1871.
Clark E. A., King W. G., Brugge J. S., Symons M., and Hynes R. O. (1998) Integrin-mediated signals regulated by members of the Rho family of GTPases. J. Cell. Biol. 142, 573–586.
Ren X.-D., Kiosses W. B., and Schwartz M. A. (1999) Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 18, 101–108.
Chrzanowska-Wodnicka M. and Burridge K. (1996) Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell. Biol. 133, 1403–1415.
Burridge K. and Chrzanowska-Wodnicka M. (1996) Focal adhesions, contractility, and signaling. Annu. Rev. Cell Div. Biol. 12, 463–519.
Fox J. E. B., Taylor R. G., Taffarel M., Boyles J. K., and Goll D. E. (1993) Evidence that activation of platelet calpain is induced as a consequence of binding of adhesive ligand to the integrin, glycoprotein IIb-IIIa. J. Cell Biol. 120, 1501–1507.
Saido T. C., Suzuki H., Yamazaki H., Tanoue K., and Suzuki K. (1993) In situ capture of μ-calpain activation in platelets. J. Biol. Chem. 268, 7422–7426.
Beckerle M. C., Burridge K., DeMartino G. N., and Croall D. E. (1987) Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion. Cell 51, 569–577.
Huttenlocher A., Palecek S. P., Lu Q., Zhang W., Mellgren R. L., Lauffenburger D. A, Ginsberg M. H., and Horwitz A. F. (1997) Regulation of cell migration by the calcium-dependent protease calpain. J. Biol. Chem. 272, 32,719–32,722.
Potter D. A., Tirnauer J. S., Janssen R., Croall D. E., Hughes C. N., Fiacco K. A., Mier J. W., Maki M., and Herman I. M. (1998) Calpain regulates actin remodeling during cell spreading. J. Cell Biol. 141, 1–16.
Lane R. D., Allan D. M., and Mellgren R. L. (1992) A comparison of the intracellular distribution of μ-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp. Cell Res. 14, 549–556.
Phillips D. R. and Agin P. P. (1977) Platelet membrane defects in Glanzmann’s thrombasthenia. J. Clin. Invest. 60, 535–545.
Schmaier A. H., Bradford H. N., Lundberg D., Farber A., and Colman R. W. (1990) Membrane expression of platelet calpain. Blood 75, 1273–1281.
Kulkarni S., Saido T. C., Suzuki K., and Fox J. E. B. (1999) Calpain mediates integrin-induced signaling at a point upstream of Rho family members. J. Biol. Chem. 274, 21,265–21,275.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Kulkarni, S., Fox, J.E.B. (2000). Localization of Calpain by Immunofluorescence in Adherent Cells. In: Elce, J.S. (eds) Calpain Methods and Protocols. Methods in Molecular Biology™, vol 144. Humana Press. https://doi.org/10.1385/1-59259-050-0:151
Download citation
DOI: https://doi.org/10.1385/1-59259-050-0:151
Publisher Name: Humana Press
Print ISBN: 978-0-89603-632-1
Online ISBN: 978-1-59259-050-6
eBook Packages: Springer Protocols