Abstract
Pulsed-field gel electrophoresis (PFGE) has been used successfully to generate physical maps of a large region from many genomes. In addition, PFGE is useful for determining the order of genes or markers more precisely than is possible with genetic linkage analysis. Since a book in the Methods in Molecular Biology series (1) has already been devoted to this subject, the aim of this chapter is to give protocols that were successfully used in our laboratory for the human mucin genes. Whenever possible, we refer to the relevant chapters of this book or other references in which strat-egies or techniques are discussed in detail.
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References
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Porchet, N., Aubert, JP. (2000). Southern Blot Analysis of Large DNA Fragments. In: Corfield, A.P. (eds) Glycoprotein Methods and Protocols. Methods in Molecular Biology™, vol 125. Humana Press. https://doi.org/10.1385/1-59259-048-9:313
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DOI: https://doi.org/10.1385/1-59259-048-9:313
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