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Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane

  • Protocol
Amino Acid Analysis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 159))

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Abstract

There are several highly sensitive methods for identifying proteins: N-ter-minal sequencing, peptide mapping, and Western blotting, which are based on partial information about the structure of the protein. The amino acid composition of a protein is reflected in the primary structure of the whole protein, and this information is highly conserved.

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References

  1. Shindo N., Nojima S., Fujimura T., Taka H., Mineki R., and Murayama K. (1997) Separation of 18 6-aminoquinolyl-carbamyl-amino acids by ion-pair chro-matography. Anal. Biochem. 249, 79–82.

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Author information

Authors and Affiliations

  1. Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan

    Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno & Kimie Murayama

Authors
  1. Noriko Shindo
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  2. Tsutomu Fujimura
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  3. Saiko Kazuno
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  4. Kimie Murayama
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Editor information

Editors and Affiliations

  1. Proteome Systems Ltd, North Ryde, Australia

    Catherine Cooper , Nicolle Packer  & Keith Williams ,  & 

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© 2001 Humana Press Inc.

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Shindo, N., Fujimura, T., Kazuno, S., Murayama, K. (2001). Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane. In: Cooper, C., Packer, N., Williams, K. (eds) Amino Acid Analysis Protocols. Methods in Molecular Biology™, vol 159. Humana Press. https://doi.org/10.1385/1-59259-047-0:087

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  • DOI: https://doi.org/10.1385/1-59259-047-0:087

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-656-7

  • Online ISBN: 978-1-59259-047-6

  • eBook Packages: Springer Protocols

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