Abstract
The amino acids we have the most experience with are GABA, glutamate and arginine, though other amino acids can be measured. The method used is based on a derivatization reported by Jacobs (1) using o-phthalaldehyde in the presence of sulphite. The method was used by Pearson et al. (2), Smith and Sharp (3), and was further developed by Rowley et al. (4).
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References
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Smith S. and Sharp T, in vivo measurement of extracellular GAB A in rat nucleus accumbens: improved methodology. Br. J. Pharmacol. 107, 210P
Rowley H. L., Martin K. F., and Marsden C. A. (1995) Determination of in vivo amino acid neurotransmitters by high-performance liquid chromatography with o-phthalaldehyde-sulphite derivatisation and electrochemical detection. J. Neurosci. Methods 57, 93–99.
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© 2001 Humana Press Inc.
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David Forster, C., Marsden, C.A. (2001). The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection. In: Cooper, C., Packer, N., Williams, K. (eds) Amino Acid Analysis Protocols. Methods in Molecular Biology™, vol 159. Humana Press. https://doi.org/10.1385/1-59259-047-0:055
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DOI: https://doi.org/10.1385/1-59259-047-0:055
Publisher Name: Humana Press
Print ISBN: 978-0-89603-656-7
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