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Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 159))

Abstract

Over the past 20 years, amino acid analysis methods based on precolumn derivatization procedures have become a popular alternative to the traditional postcolumn derivatization techniques based on ion-exchange separation of the underivatized amino acids. The major reasons for this include greater sensitivity, faster analyses, and the ability to use less expensive, more flexible HPLC instrumentation rather than dedicated amino acid analyzers.

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References

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© 2001 Humana Press Inc.

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Cohen, S.A. (2001). Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate. In: Cooper, C., Packer, N., Williams, K. (eds) Amino Acid Analysis Protocols. Methods in Molecular Biology™, vol 159. Humana Press. https://doi.org/10.1385/1-59259-047-0:039

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  • DOI: https://doi.org/10.1385/1-59259-047-0:039

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-656-7

  • Online ISBN: 978-1-59259-047-6

  • eBook Packages: Springer Protocols

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