Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization

Part of the Methods in Molecular Biology™ book series (MIMB, volume 159)

Abstract

Amino acid analysis is an important technique that has many applications in biochemical, pharmaceutical, and biomedical fields. Profiling of plasma amino acids is also of great interest in clinical practice. Many diseases are known to be associated with disorders in amino acid metabolism. The analysis of amino acids offers the possibility of genetic prevention in both premarital and prenatal stages for those diseases resulting from inborn errors of metabolism. The plasma amino acid pattern has been also used to follow the course of prolonged dietary treatment (1,2).

Keywords

HPLC Acetonitrile Heparin Adduct Sodium Hydroxide 

References

  1. 1.
    Deyl Z., Hyanek J., and Horakova M. (1986) Profiling of amino acids in body fluids and tissues by means of liquid chromatography. J. Chromatog. 379, 177–250.CrossRefGoogle Scholar
  2. 2.
    Qureshi G. A. and Qureshi A. R. (1989) Determination of free amino acids in biological samples: problems of quantitation. J. Chromatog. 491, 281–289.CrossRefGoogle Scholar
  3. 3.
    Williams A. P. (1987) General problems associated with the analysis of amino acids by automated ion-exchange chromatography. J. Chromatog. 373, 175–190.CrossRefGoogle Scholar
  4. 4.
    Fekkes D. (1996) State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples. J. Chromatog. B. Biomed. Appl. 682, 3–22.CrossRefGoogle Scholar
  5. 5.
    Uhe A. M., Collier G. R., McLennan E. A., Tucker D. J and O’Dea K. (1991) Quantitation of tryptophan and other plasma amino acids by automated pre-column o-phthaldialdehyde derivatization high-performance liquid chromatography: im-proved sample preparation. J. Chromatog. 564, 81–91.CrossRefGoogle Scholar
  6. 6.
    Sarwar G. and Botting H. G. (1993) Evaluation of liquid chromatographic analysis of nutritionally important amino acids in food and physiological samples. J. Chromatog. 615, 1–22.CrossRefGoogle Scholar
  7. 7.
    Furst P., Pollack L., Graser T. A., Godel H., and Stehle P. (1990) Appraisal of four pre-column derivatization methods for the high-performance liquid chromatographic determination of free amino acids in biological materials. J. Chromatog. 499, 557–569.CrossRefGoogle Scholar
  8. 8.
    Worthen H. G. and Liu H. (1992) Automatic pre-column derivatization and reversed-phase high performance liquid chromatography of primary and secondary amino acids in plasma with photo-diode array and fluorescence detection. J. Liq. Chromatogr. 15, 3323–3341.CrossRefGoogle Scholar
  9. 9.
    Carducci C., Birarelli M., Leuzzi V., Santagata G., Serafini P., and Antonozzi I. (1996) Automated method for the measurement of amino acids in urine by high-performance liquid chromatography. J. Chromatog. A. 729, 173–180.CrossRefGoogle Scholar
  10. 10.
    Fekkes D., van Dalen A., Edelman M., and Voskuilen A. (1995) Validation of the determination of amino acids in plasma by high-performance liquid chromatography using automated pre-column derivatization with o-phthaldialdehyde. J. Chromatog. B. Biomed. Appl. 669, 177–186.CrossRefGoogle Scholar
  11. 11.
    Liu H. and Worthen H. G. (1992) Measurement of free amino acid levels in ultrafiltrates of blood plasma by high-performance liquid chromatography with automatic pre-column derivatization. J. Chromatog. 579, 215–224.CrossRefGoogle Scholar
  12. 12.
    Terrlink T., van L. P., and Houdijk A. (1994) Plasma amino acids determined by liquid chromatography within 17 minutes. Clin. Chem. 40, 245–249.PubMedGoogle Scholar
  13. 13.
    van Eijk E. H., Rooyakkers D. R., and Deutz N. E. (1993) Rapid routine determination of amino acids in plasma by high-performance liquid chromatography with a 2–3 microns Spherisorb ODS II column. J. Chromatog. 620, 143–148.CrossRefGoogle Scholar
  14. 14.
    Georgi G., Pietsch C., and Sawatzki G. (1993) High-performance liquid chromatographic determination of amino acids in protein hydrolysates and in plasma using automated pre-column derivatization with o-phthaldialdehyde/2-mercaptoethanol. J. Chromatog. 613, 35–42.Google Scholar
  15. 15.
    Liu H., Liu Z. M., Zhu W. N., Li Y. H., Wang S. D., and Jiang X. S. (1984) Analysis of free amino acids in blood plasma by reversed phase high performance liquid chromatography with gradient elution and fluorescence detection. Chinese J. Chromatog. 1, 83–87.Google Scholar
  16. 16.
    Ersser R. S. and Davey J. F. (1991) Liquid chromatographic analysis of amino acids in physiological fluids: recent advances. Med. Lab. Sci. 48, 59–71.PubMedGoogle Scholar
  17. 17.
    Jones B. N. and Gilligan J. P. (1983) o-Phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids. J. Chromatog. 266, 471–482.CrossRefGoogle Scholar
  18. 18.
    Schuster R. (1988) Determination of amino acids in biological, pharmaceutical, plant and food samples by automated precolumn derivatization and high-performance liquid chromatography. J. Chromatog. 431, 271–284.CrossRefGoogle Scholar
  19. 19.
    Turnell D. C. and Cooper J. D. (1982) Rapid assay for amino acids in serum or urine by pre-column derivatization and reversed-phase liquid chromatography. Clin. Chem. 28, 527–531.PubMedGoogle Scholar
  20. 20.
    Ogden G. and Foldi P. (1984) Amino acid analysis: An overview of current methods. LC-GC 5, 28–40.Google Scholar
  21. 21.
    Lindroth P. and Mopper K. (1979) High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-Phthaldialdehyde. Anal. Chem. 51, 1667–1674.CrossRefGoogle Scholar
  22. 22.
    Einarsson S., Josefsson B., and Lagerkvist S. (1983) Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. J. Chromatog. 282, 609–618.CrossRefGoogle Scholar
  23. 23.
    Einarsson S. (1985) Selective determination of secondary amino acids using precolumn derivatization with 9-fluorenylmethylchloroformate and reversed-phase high-performance liquid chromatography. J. Chromatog. 348, 213–220.CrossRefGoogle Scholar
  24. 24.
    Betner I. and Foldi P. (1988) The FMOC-ADAM approach to amino acid analysis. LC-GC 6, 832–840.Google Scholar
  25. 25.
    Godel H., Graser T., Foldi P., Pfaender P., and Furst P. (1984) Measurement of free amino acids in human biological fluids by high-performance liquid chromatography. J. Chromatog. 297, 49–61.CrossRefGoogle Scholar
  26. 26.
    Blundell G. and Brydon W. G. (1987) High performance liquid chromatography of plasma aminoacids using orthophthalaldehyde derivatisation. Clin. Chim. Acta 170, 79–83.PubMedCrossRefGoogle Scholar
  27. 27.
    Feste A. S. (1992) Reversed-phase chromatography of phenylthiocarbamyl amino acid derivatives of physiological amino acids: an evaluation and a comparison with analysis by ion-exchange chromatography. J. Chromatog. 574, 23–34.CrossRefGoogle Scholar
  28. 28.
    Armstrong M. D. and Stave U. (1973) Astudy of plasma free amino acid levels. II. Normal values for children and adults. Metabolism 22, 561–569.PubMedCrossRefGoogle Scholar
  29. 29.
    Armstrong M. D. and Stave U. (1973) A study of plasma free amino acid levels. III. Variation during growth and aging. Metabolism 22, 571–578.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Hua Liu
    • 1
  1. 1.Department of ResearchTexas Scottish Rite Hospital for ChildrenDallas

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