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Matrix Metalloproteinase Protocols pp 451–456Cite as

Cartilage Proteoglycan Release Assay

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 151))

Abstract

The proteoglycans are a group of macromolecules characterized by the covalent attachment of one or more sulfated glycosaminoglycan (GAG) chains to a protein core, and are an important constituent of the extracellular matrix. Cartilage contains a number of proteoglycans, the most abundant of which is aggrecan. This proteoglycan is characterized by its association with hyaluronic acid and link protein to form large macromolecular aggregates within the tissue. The large negative charge associated with the sulfated GAGs of these aggregates serves to draw water molecules into the tissue, thus giving cartilage its ability to resist compression under load (1).

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References

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Author information

Authors and Affiliations

  1. Department of Rheumatology, The Medical School, University of Newcastle, Framlington Place, Newcastle, UK

    Caron J. Billington

Authors
  1. Caron J. Billington
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Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich, UK

    Ian M. Clark PhD

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© 2001 Humana Press Inc.

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Cite this protocol

Billington, C.J. (2001). Cartilage Proteoglycan Release Assay. In: Clark, I.M. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology™, vol 151. Humana Press. https://doi.org/10.1385/1-59259-046-2:451

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  • DOI: https://doi.org/10.1385/1-59259-046-2:451

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-733-5

  • Online ISBN: 978-1-59259-046-9

  • eBook Packages: Springer Protocols

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