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Assay of Matrix Metalloproteinases Against Matrix Substrates

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Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 151))

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Abstract

Mammalian collagenases cleave all three polypeptide chains of the triple helical collagen molecule at a specific site to give characteristic one-quarter and three-quarter fragments. These denature at 37°C becoming susceptible to digestion by less specific proteinases.

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Author information

Authors and Affiliations

  1. Department of Rheumatology, School of Clinical and Medical Sciences, University of Newcastle, UK

    Timothy E. Cawston & Andrew D. Rowan

  2. Department of Rheumatology, School of Clinical and Medical Sciences, University of Newcastle, Framlington Place, Newcastle, UK

    Paul Koshy

Authors
  1. Timothy E. Cawston
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  2. Paul Koshy
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  3. Andrew D. Rowan
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Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich, UK

    Ian M. Clark PhD

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© 2001 Humana Press Inc.

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Cawston, T.E., Koshy, P., Rowan, A.D. (2001). Assay of Matrix Metalloproteinases Against Matrix Substrates. In: Clark, I.M. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology™, vol 151. Humana Press. https://doi.org/10.1385/1-59259-046-2:389

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  • DOI: https://doi.org/10.1385/1-59259-046-2:389

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-733-5

  • Online ISBN: 978-1-59259-046-9

  • eBook Packages: Springer Protocols

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