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Purification of MMPs and TIMPs

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Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 151))

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Abstract

Currently more than twenty different matrix metalloproteinases (MMPs) and four tissue inhibitors of metalloproteinases (TIMPs) have been identified (1,2) by cDNA cloning. This chapter describes methods to purify collagenases (MMP-1, MMP-8, and MMP-13), gelatinases (MMP-2 and MMP-9), stromelysins (MMP-3 and MMP-10), macrophage elastase (MMP-12), and TIMP-1 and TIMP-2 from natural sources.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS

    Ken-ichi Shimokawa

  2. The Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, Hammersmith, London, UK

    Hideaki Nagase

Authors
  1. Ken-ichi Shimokawa
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  2. Hideaki Nagase
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Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich, UK

    Ian M. Clark PhD

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© 2001 Humana Press Inc.

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Shimokawa, Ki., Nagase, H. (2001). Purification of MMPs and TIMPs. In: Clark, I.M. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology™, vol 151. Humana Press. https://doi.org/10.1385/1-59259-046-2:275

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  • DOI: https://doi.org/10.1385/1-59259-046-2:275

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-733-5

  • Online ISBN: 978-1-59259-046-9

  • eBook Packages: Springer Protocols

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