Measuring Transcription of Metalloproteinase Genes

Nuclear Run-Off Assay vs Analysis of hnRNA
  • Anne M. Delany
Part of the Methods in Molecular Biology™ book series (MIMB, volume 151)


Many metalloproteinase genes are transcriptionally regulated by differentiation, growth factors, cytokines, and hormones. Nuclear run-off assays and analysis of heterogeneous nuclear RNA (hnRNA) levels are two methods for studying changes in gene transcription. In this chapter these two techniques will be outlined and compared.


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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Anne M. Delany
    • 1
  1. 1.University of Connecticut School of MedicineFarmington

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