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Measuring Transcription of Metalloproteinase Genes

Nuclear Run-Off Assay vs Analysis of hnRNA
  • Anne M. Delany
Part of the Methods in Molecular Biology™ book series (MIMB, volume 151)

Abstract

Many metalloproteinase genes are transcriptionally regulated by differentiation, growth factors, cytokines, and hormones. Nuclear run-off assays and analysis of heterogeneous nuclear RNA (hnRNA) levels are two methods for studying changes in gene transcription. In this chapter these two techniques will be outlined and compared.

Keywords

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Anne M. Delany
    • 1
  1. 1.University of Connecticut School of MedicineFarmington

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