Rapid Identification of Bacteria Based on Spectral Patterns Using MALDI-TOFMS

  • Jackson O. LayJr.
  • Ricky D. Holland
Part of the Methods in Molecular Biology™ book series (MIMB, volume 146)


The characterization of whole or “intact” bacteria, based on the mass spectral detection of genus, species, phenotypic, or genotypic biomarkers, desorbed and ionized directly without any isolation, purification, or concentration steps, has been accomplished using matrix-assisted laser/desorption ionization (MALDI) coupled with time-of-flight mass spectrometry (TOF-MS). Because MALDI-TOFMS plays such a key role in this method and also because these techniques may be unfamiliar to some readers, brief introductions to TOF-MS and MALDI and their advantages for bacterial characterization are presented in Subheadings 1.1. and 1.2.


Reference Spectrum Enterobacter Cloaca Sinapinic Acid Shigella Flexneri MALDI Matrix 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Jackson O. LayJr.
    • 1
  • Ricky D. Holland
    • 2
  1. 1.Division of ChemistryNational Center for Toxicological Research, Food and Drug AdministrationJefferson
  2. 2.Division of ChemistryNational Center for Toxicological Research, Food and Drug AdministrationJefferson

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