Abstract
Intercellular gap junction (GJ) channels arise from the association of two hemichannels, ahexameric assembly of connexin membrane proteins, between two cells (for review, see refs. 1,2). Connexins have the shortest half-lives of all known membrane channel proteins, approx 2-3 h, and consequently GJ channel formation and turnover is believed to be relatively fast (for review, see ref. 3).
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References
Bennett M. V. and Verselis V. K. (1992) Biophysics of gap junctions. Semin. Cell Biol. 3, 29–47.
Sosinsky G. E. (1996) Molecular organization of gap junction membrane channels. J. Bioenerg. Biomembr. 28, 297–309.
Laird D. W. (1996) The life cycle of a connexin: gap junction formation, removal, and degradation. J. Bioenerg. Biomembr. 28, 311–318.
Loewenstein W. R., Kanno Y., and Socolar S. J. (1978) Quantum jumps of conductance during formation of membrane channels at cell-cell junction. Nature 274, 133–136.
Chow I. and Poo M. M. (1984) Formation of electrical coupling between embryonic Xenopus muscle cells in culture. J. Physiol. 346, 181–194.
Chow I. and S H. Young (1987) Opening of single gap junction channels during formation of electrical coupling between embryonic muscle cells. Dev. Biol. 122, 332–337.
Rook M. B., Jongsma H. J., and van Ginneken A. C. ( 1988) Properties of single gap junctional channels between isolated neonatal rat heart cells. Am. J. Physiol. 255, H770–H782.
Churchill D. and Caveney S. (1993) Double whole-cell patch-clamp characterization of gap junctional channels in isolated insect epidermal cell pairs. J. Membr. Biol. 135, 165–180.
Bukauskas F. F. and Weingart R. (1993) Multiple conductance states of newly formed single gap junction channels between insect cells. Pflügers Arch. 423, 152–154.
Bukauskas F. F., Elfgang C., Willecke K., and Weingart R. (1995) Heterotypic gap junction channels (connexin26-connexin32) violate the paradigm of unitary conductance. Pflügers Arch. 429, 870–872.
Bukauskas F. F., Elfgang C., Willecke K., and Weingart R. (1995) Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys. J. 68, 2289–2298.
Keane R. W., Mehta P. P., Rose B., Honig L. S., Loewenstein W. R., and Rutishauser U. (1988) Neural differentiation, NCAM-mediated adhesion, and gap junctional communication in neuroectoderm. A study in vitro. J. Cell Biol. 106, 1307–1319.
Meyer R. A., Laird D. W., Revel J. P., and Johnson R. G. (1992) Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies. J. Cell Biol. 119, 179–189.
Ghoshroy S., Goodenough D. A., and Sosinsky G. E. (1995) Preparation, characterization, and structure of half gap junctional layers split with urea and EGTA. J. Membr. Biol. 146, 15–28.
Johnson R., Meyer R., and Lampe P. (1989) Gap junction formation: A self-assembly model involving membrane domains of lipid and protein, in Cell Interaction and Gap Junctions (Sperelakis N. and Cole W. C., eds.), CRC Press, Boca Raton, FL, pp. 159–179.
Burt J. M. and Spray D. C. (1988) Inotropic agents modulate gap junctional conductance between cardiac myocytes. Am. J. Physiol. 254, H1206–H1210.
Giaume C., C. Randriamampita, and A. Trautmann (1989) Arachidonic acid closes gap junction channels in rat lacrimal glands. Pflügers. Arch. 413, 273–279.
Bukauskas F. F. and Weingart R. (1994) Voltage-dependent gating of single gap junction channels in an insect cell line. Biophys. J. 67, 613–625.
Bukauskas F. F., Vogel R., and Weingart R. (1997) Biophysical properties of heterotypic gap junctions newly formed between two types of insect cells. J. Physiol. (London) 499(Pt 3), 701–713.
Valiunas V., Bukauskas F. F., and Weingart R. (1997) Conductances and selective permeability of connexin43 gap junction channels examined in neonatal rat heart cells. Circ. Res. 80, 708–719.
Bukauskas F. F., Shrager P., and Peracchia C. (1998) Gating properties of gap junction channels in Schwann cells and fibroblasts isolated from the sciatic nerve of neonatal rats, in Gap Junctions (Werner R., ed.), IOS Press, Amsterdam, pp. 25–29.
Churchill D., Coodin S., Shivers R. R., and Caveney S. (1993) Rapid de novo formation of gap junctions between insect hemocytes in vitro—A freeze-fracture, dye-transfer and patch-clamp study. J. Cell Sci. 104, 763–772.
Bukauskas F. F. and Weingart R. (1993) Temperature dependence of gap junction properties in neonatal rat heart cells. Pflügers Arch. 423, 133–139.
Elfgang C., Eckert R., Lichtenberg-Frate H., Butterweck A., Traub O., Klein R. A., Hulser D. F., and Willecke K. (1995) Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J. Cell. Biol. 129, 805–817.
Neyton J. and Trautmann A. (1985) Single-channel currents of an intercellular junction. Nature 317, 331–335.
Weingart R. and Bukauskas F. F. (1993) Gap junction channels of insects exhibit a residual conductance. Pflügers Arch. 424, 192–194.
Cao F., Eckert R., Elfgang C., Nitsche J. M., Snyder S. A., Hulser D. F., Willecke K., and Nicholson B. J. (1998) A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes. J. Cell Sci. 111, 31–43.
Barrio L. C., Suchyna T., Bargiello T., Xu L. X., Roginski R. S., Bennett M. V., and Nicholson B. J. (1991) Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. Proc. Natl. Acad. Sci. USA 88, 8410–8414.
Bruzzone R., White T. W., and Paul D. L. (1994) Expression of chimeric connexins reveals new properties of the formation and gating behavior of gap junction channels. J. Cell Sci. 107, 955–967.
Bruzzone R., Haefliger J. A., Gimlich R. L., and Paul D. L. (1993) Connexin 40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol. Biol. Cell 4, 7–20.
White T. W., Bruzzone R., Wolfram S., Paul D. L., and Goodenough D. A. (1994) Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins. J. Cell Biol. 125, 879–892.
Werner R., Levine E., Rabadan Diehl C., and Dahl G. (1989) Formation of hybrid cell-cell channels. Proc. Natl. Acad. Sci. USA 86, 5380–5384.
White T. W., Paul D. L., Goodenough D. A., and Bruzzone R. (1995) Functional analysis of selective interactions among rodent connexins. Mol. Biol. Cell 6, 459–470.
Bukauskas F. F. and Weingart R. (1995) Gating properties of homo-and heterotypic gap junction channels formed by different mouse connexins, in Proceedings of the 1995 Gap Junction Conference, Ile des Embiez, France.
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Bukauskas, F.F. (2001). Inducing De Novo Formation of Gap Juntion Channels. In: Bruzzone, R., Giaume, C. (eds) Connexin Methods and Protocols. Methods In Molecular Biology™, vol 154. Humana Press. https://doi.org/10.1385/1-59259-043-8:379
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DOI: https://doi.org/10.1385/1-59259-043-8:379
Publisher Name: Humana Press
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