Abstract
By the mid 1960s, pioneering work using high-resolution electron microscopy, new fixation methods, and negative staining of isolated liver plasma membranes allowed the identification of a geometric subunit pattern likely associated with junctional domains (1,2). Furthermore, the application of tissue impregnation with electron-dense tracers revealed that the minute “gap” (2 nm wide) between the closely adjoining junctional membranes comprised an hexagonal subunit pattern. This type of membrane-membrane interaction, distinct from tight junctions, adhesion plaques, and desmosomes, was originally called “gap junction” (3).
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Dunia, I., Recouvreur, M., Nicolas, P., Kumar, N.M., Bloemendal, H., Benedetti, E.L. (2001). Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions. In: Bruzzone, R., Giaume, C. (eds) Connexin Methods and Protocols. Methods In Molecular Biology™, vol 154. Humana Press. https://doi.org/10.1385/1-59259-043-8:33
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DOI: https://doi.org/10.1385/1-59259-043-8:33
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