Abstract
Energy homeostasis is the process by which adipose tissue, the stored body energy, is kept constant over time. Obviously, feeding behavior plays a crucial role in energy homeostasis. Therefore, to maintain a constant energy balance, energy (food) intake should match energy expenditure. A number of humoral factors are involved in signaling the body need in energy to modulate food intake. Among these hormones, leptin and insulin are secreted proportionally to the amount of body fat. They act in the central nervous system to regulate the action and expression of a variety of neurotransmitters and then modify energy balance by changing food intake and weight gain. The neurotransmitter neuropeptide Y (NPY) is a key player in the modulation of the leptin signal (1). In particular, NPY promotes weight gain via its stimulatory effect on food intake in the hypothalamus. In addition, more evidence suggests that NPY also acts on specific target organs at the periphery to modify body fat stores. At least five different NPY receptor subtypes have been identified (2). It has been hypothesized that each of these receptors might be associated with specific NPY functions.
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References
Stephens T. W., Basinski M., Bristow P. K., Bue-Valleskey J. M., Burgett S. G., Craft L., et al. (1995) The role of neuropeptide Y in the antiobesity action of the obese gene product. Nature 377, 530–532.
Blomqvist A. G. and Herzog H. (1997) Y-receptor subtypes: how many more? Trends Neurosci. 20, 294–298.
Pedrazzini T., Seydoux J., Künstner P., Aubert J. F., Grouzmann E., Beermann F., and Brunner H.-R. (1998) Cardiovascular response, feeding behaviour and locomotor activity in mice lacking the NPY Y1 receptor. Nature Med. 4, 722–726.
Joyner A. L. (1993) in Gene Targeting (Rickwood D. and Hames B. D., eds.), Oxford University Press Oxford.
Entenman C. (1957) in Methods in Enzymology (Colowick S. P. and Kaplan O.,eds.), Academic New York, pp. 299–317.
Selfridge J., Pow A. M., McWhir J., Magin T. M., and Melton D. W. (1992)Gene targeting using the mouse HPRT minigene/HPRT-deficient embryonic stem cell system: inactivation of the mouse ERCC-1 gene. Somat. CellMol. Genet. 18, 325–336.
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© 2000 Humana Press Inc.
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Pedrazzini, T., Seydoux, J. (2000). NeuropeptideYY1 Receptor-Deficient Mice. In: Balasubramaniam, A. (eds) Neuropeptide Y Protocols. Methods in Molecular Biology™, vol 153. Humana Press. https://doi.org/10.1385/1-59259-042-X:91
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DOI: https://doi.org/10.1385/1-59259-042-X:91
Publisher Name: Humana Press
Print ISBN: 978-0-89603-662-8
Online ISBN: 978-1-59259-042-1
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