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Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs

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The Nucleic Acid Protocols Handbook

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Abstract

Most attempts to identify and isolate a novel cDNA result in the acquisition of clones that represent only a part of the mRNA’s complete sequence (Fig. 1). The approach described here to clone the missing sequence (cDNA ends) employs polymerase chain reaction (PCR). Since the initial reports of rapid amplification of cDNA ends (RACE) (1) or related techniques (2,3), many labs have developed significant improvements on the basic approach (4–18). The most recent hybrid version of the relatively simple Classic RACE will be described here, as well as a more powerful but technically more challenging “New RACE” protocol, which is adapted from the work of a number of laboratories (19–26). Commercial RACE kits are available from Bethesda Research Laboratories (Gaithersburg, MD) (11) and Clontech (Palo Alto, CA) that are convenient but not as powerful as the most recent versions of Classic and New RACE.

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Authors and Affiliations

  1. Department of Pharmacology, University Medical Center at Stony Brook, Stony Brook, NY

    Yue Zhang & Michael A. Frohman

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  1. Yue Zhang
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  2. Michael A. Frohman
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Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    Ralph Rapley

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© 2000 Humana Press Inc., Totowa, NJ

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Zhang, Y., Frohman, M.A. (2000). Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:267

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  • DOI: https://doi.org/10.1385/1-59259-038-1:267

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-459-4

  • Online ISBN: 978-1-59259-038-4

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