Skip to main content

Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence

  • Protocol
The Nucleic Acid Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 146 Accesses

Abstract

The use of enzyme-labeled probes, in conjunction with chemiluminescence, allows a flexible approach to hybridization and detection procedures. This is shown by the diversity of applications in which the system can be used, including Southern blots (1,2), Northern blots (3), colony and plaque screening (4), yeast artificial chromosome clone screening (5), and polymerase chain reaction product detection (6,7).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Heslop-Harrison, J. S. (1990) Gene expression and parental dominance in hybrid plants. Development (suppl.), 21–28.

    Google Scholar 

  2. Tonjes, R. R. (1991) Detecting transgene sequences by Southern blot analysis using the ECL gene detection system. Life Sci. News 4, 7–8.

    Google Scholar 

  3. Einspanier, R. and Scham, D. (1991) Determination of insulin-like growth factor I and II in bovine ovarian follicles and cysts. Acta Endocrinol. 124(suppl. 1), 19.

    Google Scholar 

  4. Durrant, I., Benge, L. C. A., Sturrock, C., Devenish, A. T., Howe, R., Roe, S., Moore, M., Scozzafava, G., Proudfoot, L. M. F., Richardson, T. C, and McFarthing, K. G. (1990) The application of enhanced chemiluminescence to membrane based nucleic acid detection. Biotechniques 8, 564–570.

    PubMed  CAS  Google Scholar 

  5. Stone, T. and Durrant, I. (1992) Enhanced chemiluminescence for the detection of bound nucleic acid sequences. Genet. Anal.: Tech. Appl. 8, 230–237.

    Google Scholar 

  6. Sorg, R., Enczmann, J., Sorg, U., Kogler, G., Schneider, E. M., and Wernet, P. (1990) Specific non-radioactive detection of PCR-amplified sequences with enhanced chemiluminescence labeling. Life Sci. News 2, 3–4.

    Google Scholar 

  7. Tucker, S. J. (1991) ECL direct system: identification of the cystic fibrosis gene of Xenopus laevis. Highlights 2, 4.

    Google Scholar 

  8. Hutton, J. R. (1977) Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea. Nucleic Acids Res. 4, 3537–3555.

    Article  PubMed  CAS  Google Scholar 

  9. Britten, R. J. and Davidson, E. H. (1985) Hybridization strategy, in Nucleic Acid Hybridization: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL Press, Oxford, pp. 3–15.

    Google Scholar 

  10. Stone, T. (1992) ECL direct system. An analysis of filter hybridization kinetics. Life Sci. News 7, 8.

    Google Scholar 

  11. Durrant, I. (1990) Light based detection of biomolecules. Nature (Lond.) 346, 297–298.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Stone, T., Durrant, I. (2000). Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:157

Download citation

  • DOI: https://doi.org/10.1385/1-59259-038-1:157

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-459-4

  • Online ISBN: 978-1-59259-038-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics