Native Polyacrylamide Gel Electrophoresis

  • Adrian J. Harwood

Abstract

Agarose gel electrophoresis is generally adequate for resolving nucleic acid fragments in the size range of 100 nucleotides to around 10–15 kb (see  Chapter 13). Below this range, fragments are both difficult to separate and hard to visualize because of diffusion within the gel matrix. These problems are solved by native polyacrylamide gel electrophoresis (PAGE). Using native PAGE, fragments as small as 10 bp and up to 1 kb can be separated with a resolution of as little as 1 bp.

Keywords

Vortex EDTA Electrophoresis Dimethyl Polyacrylamide 

Reference

  1. 1.
    Maxam, A. M. and Gilbert, W. (1977) A new method for sequencing DNA. Proc. Natl. Acad. Sci. USA 73, 668–671.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Adrian J. Harwood
    • 1
  1. 1.MRC Laboratory for Molecular Cell BiologyUniversity College LondonLondonUK

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