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Restriction Fragment Length Polymorphism

  • Mohammad S. Enayat

Abstract

DNA sequence changes within a gene results either in polymorphism or mutation causing different diseases. Some of these polymorphisms that occur with a high frequency within the population can be a useful tool for gene tracking for a given disease. Such investigations have initially been done by Southern blot techniques, but where possible have now been replaced by polymerase chain reaction (PCR)-based methodology. The nucleotide substitutions can be identified in two ways:
  1. 1.

    By use of restriction enzyme analysis or restriction fragment length polymorphisms (RFLP).

     
  2. 2.

    Allele specific oligonucleotide hybridization (ASO-H) or similar techniques.

     

Keywords

Restriction Fragment Length Polymorphism Variable Number Tandem Repeat Trisodium Citrate Restriction Fragment Length Polymorphism Neutralization Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Mohammad S. Enayat
    • 1
  1. 1.Department of HaematologyBirmingham Children’s Hospital NHS TrustBirminghamUK

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