Restriction Fragment Length Polymorphism

  • Mohammad S. Enayat

Abstract

DNA sequence changes within a gene results either in polymorphism or mutation causing different diseases. Some of these polymorphisms that occur with a high frequency within the population can be a useful tool for gene tracking for a given disease. Such investigations have initially been done by Southern blot techniques, but where possible have now been replaced by polymerase chain reaction (PCR)-based methodology. The nucleotide substitutions can be identified in two ways:
  1. 1.

    By use of restriction enzyme analysis or restriction fragment length polymorphisms (RFLP).

     
  2. 2.

    Allele specific oligonucleotide hybridization (ASO-H) or similar techniques.

     

Keywords

Glycerol Albumin Recombination Agarose Citrate 

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Mohammad S. Enayat
    • 1
  1. 1.Department of HaematologyBirmingham Children’s Hospital NHS TrustBirminghamUK

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