Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
The time and labor required for generating nucleotide sequence information has been significantly reduced since the development of the polymerase chain reaction (PCR). PCR allows the production of sufficient amounts of DNA template in vitro, and consequently the time-consuming cloning procedure to obtain large enough quantities of DNA for sequencing is avoided. The PCR has transformed nucleotide sequencing into a method that can be considered even in routine diagnostics for sequencing large numbers of individual samples.
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- 4.Syvänen, A.-C., Bengtström, M., Tenhunen, J., and Söderlund, H. (1988) Quantification of polymerase chain reaction products by affinity-based hybrid collection. Nucleic Acids Res. 16,11,327-11,338.Google Scholar
- 7.Hultman, T., Bergh, S., Moks, T., and Uhlén, M. (1991) Bidirectional solid-phase sequencing of in vitro amplified plasmid DNA. Bio Techniques 10, 84–93.Google Scholar
- 11.Wu, R., Wu, N.-H., Hanna, Z., Georges, F., and Narang, S. (1984) In Oligonucleotide Synthesis: A Practical Approach (Gait, M. J., ed.), IRL Press, Oxford, UK, p. 135.Google Scholar