Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence

  • Timothy Stone
  • Ian Durrant


The use of enzyme-labeled probes, in conjunction with chemiluminescence, allows a flexible approach to hybridization and detection procedures. This is shown by the diversity of applications in which the system can be used, including Southern blots (1,2), Northern blots (3), colony and plaque screening (4), yeast artificial chromosome clone screening (5), and polymerase chain reaction product detection (6,7).


Amersham Pharmacia Biotech Wash Buffer Hybridization Buffer Standard Saline Citrate Saran Wrap 
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  1. 1.
    Heslop-Harrison, J. S. (1990) Gene expression and parental dominance in hybrid plants. Development (suppl.), 21–28.Google Scholar
  2. 2.
    Tonjes, R. R. (1991) Detecting transgene sequences by Southern blot analysis using the ECL gene detection system. Life Sci. News 4, 7–8.Google Scholar
  3. 3.
    Einspanier, R. and Scham, D. (1991) Determination of insulin-like growth factor I and II in bovine ovarian follicles and cysts. Acta Endocrinol. 124(suppl. 1), 19.Google Scholar
  4. 4.
    Durrant, I., Benge, L. C. A., Sturrock, C., Devenish, A. T., Howe, R., Roe, S., Moore, M., Scozzafava, G., Proudfoot, L. M. F., Richardson, T. C, and McFarthing, K. G. (1990) The application of enhanced chemiluminescence to membrane based nucleic acid detection. Biotechniques 8, 564–570.PubMedGoogle Scholar
  5. 5.
    Stone, T. and Durrant, I. (1992) Enhanced chemiluminescence for the detection of bound nucleic acid sequences. Genet. Anal.: Tech. Appl. 8, 230–237.Google Scholar
  6. 6.
    Sorg, R., Enczmann, J., Sorg, U., Kogler, G., Schneider, E. M., and Wernet, P. (1990) Specific non-radioactive detection of PCR-amplified sequences with enhanced chemiluminescence labeling. Life Sci. News 2, 3–4.Google Scholar
  7. 7.
    Tucker, S. J. (1991) ECL direct system: identification of the cystic fibrosis gene of Xenopus laevis. Highlights 2, 4.Google Scholar
  8. 8.
    Hutton, J. R. (1977) Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea. Nucleic Acids Res. 4, 3537–3555.PubMedCrossRefGoogle Scholar
  9. 9.
    Britten, R. J. and Davidson, E. H. (1985) Hybridization strategy, in Nucleic Acid Hybridization: A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL Press, Oxford, pp. 3–15.Google Scholar
  10. 10.
    Stone, T. (1992) ECL direct system. An analysis of filter hybridization kinetics. Life Sci. News 7, 8.Google Scholar
  11. 11.
    Durrant, I. (1990) Light based detection of biomolecules. Nature (Lond.) 346, 297–298.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Timothy Stone
    • 1
  • Ian Durrant
    • 1
  1. 1.Resarch and DevelopmentAmersham Pharmacia Biotech, Ltd.UK

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