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Critical Point Drying of Biological Specimens for Scanning Electron Microscopy

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Supercritical Fluid Methods and Protocols

Part of the book series: Methods In Biotechnologyâ„¢ ((MIBT,volume 13))

Abstract

Although several methods can be used to dry specimens for examination with the scanning electron microscope (SEM), critical point drying (CPD) is by far the most widely used. The technique was first introduced by Anderson (1) to preserve three-dimensional structure of biological specimens for transmission electron microscopy. Later, it was reintroduced (2) as a method of obtaining dry specimens for SEM examination.

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References

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© 2000 Humana Press Inc.

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Bray, D. (2000). Critical Point Drying of Biological Specimens for Scanning Electron Microscopy. In: Williams, J.R., Clifford, A.A. (eds) Supercritical Fluid Methods and Protocols. Methods In Biotechnologyâ„¢, vol 13. Humana Press. https://doi.org/10.1385/1-59259-030-6:235

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  • DOI: https://doi.org/10.1385/1-59259-030-6:235

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-571-3

  • Online ISBN: 978-1-59259-030-8

  • eBook Packages: Springer Protocols

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