Abstract
Salbutamol sulfate, shown above, is a bronchodilator used for the treatment of asthma. Most of the papers published in relation to salbutamol sulfate analysis described its determination and quantification in tissues and biological fluids of animals under treatment with this drug. Normally, the methods employed are based on high-performance liquid chromatography (HPLC) techniques using detectors of high sensitivity such as fluorescence (1–3) and electrochemical (4–6). It is known that there are some impurities that could be produced during synthesis or during storage of the drug. Consequently, there is great interest in the analysis of the drug and its impurities. HPLC (7) or capillary electrophoresis (8) methods have been used to determine these compounds, but only two of the impurities were analyzed. Recently, salbutamol sulfate and six related impurities were separated by HPLC in 30 min (9). To shorten this time, analysis of the same samples by supercritical fluid chromatography (SFC) was tried. It was found that analysis times of less than 15 min were possible (10).
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References
Degroodt J. M., Debukanski B. W., and Srebrnik S. (1992) Immunoaffinitychromatography purification of salbutamol in liver and HPLC-fluorometric detection at trace residue level. Z. Lebens 195, 566–568.
McCarthy P. T., Atwal S., Sykes A. P., and Ayres J. G. (1993) Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection. J. Biomed. Chromatogr. 7, 25–28.
Gupta R. N., Fuller H. D., and Dolovich M. B. (1994) Optimization of a column liquid chromatographic procedure for the determination of plasma salbutamol concentration. J. Chromatogr. B 654, 205–211.
Sagar K. A., Hua C., Kelly M. T., and Smyth M. R. (1992) Analysis of salbutamol in human plasma by high performance liquid chromatography with electrochemical detection using a micro electrochemical flow cell. Electroanalysis 4, 481–486.
Sagar K. A., Kelly M. T., and Smyth M. T. (1993) Simultaneous determination of salbutamol and terbutaline at overdose levels in human plasma by high performance liquid chromatography with electrochemical detection. J. Biomed. Chromatogr. 7, 29–33.
Ramos F., Castihlo M. C., Dasilveira M. I. N., Prates J. A. M., and Correira J. H. R. (1993) Determination of salbutamol in rats at low concentrations using liquid chromatography with electrochemical detection. Anal. Chim. Acta 275, 279–283.
Mulholland M. and Waterhouse J. (1988) Investigation of the limitations of saturated fractional factorial experimental designs, with confounding effects for an HPLC ruggedness test. Chromatographia 25, 769–774.
Altria K. D. (1993) Determination of salbutamol related impurities by capillary electrophoresis. J. Chromatogr. 634, 323–328.
Bernal J. L., Nozal M2. J., Velasco H., and Toribio L. (1996) HPLC versus SFC for the determination of salbutamol sulphate and its impurities in pharmaceuticals. J. Liq. Chrom. Rel. Technol. 19, 1579–1589.
Bernal J. L., Nozal M2. J., Rivera J. M., Serna M2. L., and Toribio L. (1996) Separation of salbutamol and six related impurities by packed column supercritical fluid chromatography. Chromatographia 42, 89–94.
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© 2000 Humana Press Inc.
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Nozal, M.J.d., Toribio, L., Bernal, J.L., Serna, M.L. (2000). Determination of Salbutamol Sulfate and Its Impurities in Pharmaceuticals by Supercritical Fluid Chromatography. In: Williams, J.R., Clifford, A.A. (eds) Supercritical Fluid Methods and Protocols. Methods In Biotechnology™, vol 13. Humana Press. https://doi.org/10.1385/1-59259-030-6:157
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DOI: https://doi.org/10.1385/1-59259-030-6:157
Publisher Name: Humana Press
Print ISBN: 978-0-89603-571-3
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