Abstract
The determination of trace organic compounds in aqueous samples usually involves isolation of the fraction of interest followed by subsequent separation by means of a chromatographic technique. When high resolution is needed, the main chromatographic techniques usually employed are capillary gas chromatography (GC) or supercritical fluid chromatography (SFC), both as analytical tools in themselves and as inlet methods for mass spectrometry (MS) (1). SFC (see Chapter 1) has features overlapping gas and liquid chromatography, and may use numerous detectors under mild conditions, including the universal flame ionization detector (FID) and improved chromatographic-mass spectrometric interfaces, that opens additional possibilities for the study of retinoids, carotenoids, other vitamins, and related compounds (2). One drawback of the SFC techniques when using carbon dioxide as mobile phase is that the direct introduction of water samples poses a series of problems. Water must therefore be eliminated before it reaches the analytical column. A sample preparation step is thus essential to both concentrate the sample and eliminate the water.
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References
Pinkston J. D. and Chester T. L. (1995) Guidelines for Successful SFC/MS. Anal. Chem. 67, 650A–656A.
Furr H. C., Barua A. B., and Olson J. A. (1992) Retinoids and carotenoids, in Modern Chromatographic Analysis of Vitamins (De Leenheer A. P., Lambert W. E., and Nelis H. J., eds.) Marcel Dekker, New York, pp. 38–39.
Greibrokk T. (1995) Applications of SFE in multidimensional systems. J. Chromatogr. A 703, 523–536.
Luque de Castro M. D., Valcárcel M., and Tena M. T. (1992) Analytical Supercritical Fluid Extraction. Springer-Verlag, Heidelberg, pp. 62–65.
Lee M. L. and Markides K. E. (1990) Analytical Supercritical Fluid Chromatography and Extraction. Chromatography Conferences, Provo, Utah.
Louter A. J. H., Ramalho S., Vreuls R. J. J., Jahr D., and Brinkman U. A. Th. (1996) An improved approach for on-line solid-phase extraction-gas chromatography. J. Microcol. Sep. 8, 469–477.
Riekkola M.-L., Manninen P., and Hartonen K. (1992) SFE, SFE/GC and SFE/ SFC: instrumentation and applications, in Hyphenated Techniques in Supercritical Chromatography and Extraction (Jinno K., ed.), Chromatography Library, Vol. 53. Elsevier Science, Amsterdam, pp. 275–304.
Hawthorne S. B. (1990) Analytical-scale SFE. Anal. Chem. 62, 633A–642A.
Chester T. L., Pinkston J. D., and Raynie D. E. (1996) Supercritical fluid chromatography and extraction. Anal. Chem. 68, 487R–514R.
Koski I. J., Jansson B. A., Markides K. E., and Lee M. L. (1991) Analysis of prostaglandins in aqueous solutions by supercritical fluid extraction and chromatography. J. Pharm. Biomed. Anal. 9, 281–290.
Reighard T. S. and Olesik S. V. (1996) Bridging the gap between supercritical fluid extraction and liquid extraction techniques: alternative approaches to the extraction of solid and liquid environmental matrices. Crit. Rev. Anal. Chem. 26, 61–99.
Petersson U. and Markides K. E. (1996) Stability and purity of low-polarity adsorbents for coupled supercritical fluid extraction-supercritical fluid chromatography-flame ionisation detection. J. Chromatogr. A 734, 311–318.
Sandra P., Medvedovici A., Kot A., Vilas Boas L., and David F. (1996) SPE-SFC-DAD: a new hyphenated system for monitoring organic micropollutants in aqueous samples. LC-GC Int. 9, 540–554.
Pocurull E., Marcé R. M., Borrull F., Bernal J. L., Toribio L., and Serna M. L. (1996) On-line solid-phase extraction coupled to supercritical fluid chromatography to determine phenol and nitrophenols in water. J. Chromatogr. A 755, 67–74.
Janda V., Mikesová M., and Vejrosta J. (1996) Direct supercritical fluid extraction of water-based matrices. J. Chromatogr. A 733, 35–40.
Se~noráns F. J., Petersson U., and Markides K. E. (1997) Microdialysis/SFE/ SFC/FID of antioxidants and related compounds in water, in Proceedings of the Nineteenth International Symposium on Capillary Chromatography and Electrophoresis, May 18–22,1997, Wintergreen, VA pp. 434–435.
Ullsten U. and Markides K. E. (1994) Automated on-line solid phase adsorption/ supercritical fluid extraction/supercritical fluid chromatography of analytes from polar solvents. J. Microcol. Sep. 6, 385–393.
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Se~noráns, F.J., Markides, K.E. (2000). On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices. In: Williams, J.R., Clifford, A.A. (eds) Supercritical Fluid Methods and Protocols. Methods In Biotechnology™, vol 13. Humana Press. https://doi.org/10.1385/1-59259-030-6:127
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DOI: https://doi.org/10.1385/1-59259-030-6:127
Publisher Name: Humana Press
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