Abstract
The analysis of drugs and their metabolites in biological fluids represents an essential role in pharmaceutical and toxicology studies. High performance liquid chromatography (HPLC) has emerged as a particularly powerful analytical technique for drug analysis since many water soluble compounds are too involatile and/or thermally labile to be analyzed by gas chromatography.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Howard A. L. and Taylor L. T. (1993) Supercritical fluid extraction-high performance liquid chromatography: on-line and off-line strategies, in Supercritical Fluid Extraction and Its Use in Chromatographic Sample Preparation (Westwood S. A., ed.) Blackie Academic and Professional, London, pp. 145–168.
Griebrokk T. (1995) Applications of supercritical fluid extraction in multidimensional systems. J. Chromatogr. A 703, 523–536.
Rees A. T. (1998) Supercritical fluid extraction for off-line and on-line high performance liquid chromatographic analysis, in Analytical Supercritical Fluid Extraction Techniques (Ramsey E. D., ed.) Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 330–349.
Ramsey E. D., Minty B., and Rees A. T. (1997) Drugs in water: analysis at the part-per-billion level using direct supercritical fluid extraction of aqueous samples coupled on-line with ultraviolet-visible diode-array liquid chromatography-mass spectrometry. Anal. Comm. 34, 51–54.
Saito M. and Yamauchi Y. (1994) Instrumentation, in Fractionation by Packed-Column SFC and SFE (Saito M., Yamauchi Y., and Okuyama T., eds.), VCH Publishers Inc., New York, pp. 101–133.
Taylor L. T. (1996) Supercritical Fluid Extraction. Wiley, New York, pp. 53–57.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Ramsey, E.D., Minty, B., Rees, A.T. (2000). Analysis of Anabolic Drugs by Direct Aqueous Supercritical Fluid Extraction Coupled On-Line with High-Performance Liquid Chromatography. In: Williams, J.R., Clifford, A.A. (eds) Supercritical Fluid Methods and Protocols. Methods In Biotechnologyâ„¢, vol 13. Humana Press. https://doi.org/10.1385/1-59259-030-6:113
Download citation
DOI: https://doi.org/10.1385/1-59259-030-6:113
Publisher Name: Humana Press
Print ISBN: 978-0-89603-571-3
Online ISBN: 978-1-59259-030-8
eBook Packages: Springer Protocols