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Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Multiplex Polymerase Chain Reaction

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Food Microbiology Protocols

Part of the book series: Methods in Biotechnology ((MIBT,volume 14))

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Abstract

Since 1983, when Escherichia coli O157:H7 was first recognised as a cause of hemorrhagic colitis and hemolytic uremic syndrome (HUS), verotoxin-producing strains of E. coli (VTEC) have been identified as the cause of increasing numbers of cases of serious human illness (1,2). One of a number of large outbreaks in Scotland resulted in 20 deaths, and a massive outbreak in Japan affected more than 9000 people (3,4). In England and Wales, 90 % of VTEC cases are sporadic (5).

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Authors and Affiliations

  1. Bacteriology Department, Belfast City Hospital,Belfast, Northern Ireland

    Ian G. Wilson

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  1. Ian G. Wilson
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Editor information

Editors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiologicos, San Miguel Tucumán, Argentina

    John F. T. Spencer  & Alicia L. de Ragout Spencer  & 

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© 2001 Humana Press Inc.

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Wilson, I.G. (2001). Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Multiplex Polymerase Chain Reaction. In: Spencer, J.F.T., de Ragout Spencer, A.L. (eds) Food Microbiology Protocols. Methods in Biotechnology, vol 14. Humana Press. https://doi.org/10.1385/1-59259-029-2:85

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  • DOI: https://doi.org/10.1385/1-59259-029-2:85

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-867-7

  • Online ISBN: 978-1-59259-029-2

  • eBook Packages: Springer Protocols

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