Skip to main content

Methods to Determine Proteolytic Activity of Lactic Acid Bacteria

  • Protocol
Food Microbiology Protocols

Part of the book series: Methods in Biotechnology ((MIBT,volume 14))

Abstract

Lactic acid bacteria (LAB) are considered weakly proleolytic when compared with many other groups of bacteria (e.g., Bacillus, Proteus, Pseudomonas). However, most strains of LAB rely on a complex proteolytic system that allow them to liberate essential and growth-stimulatory amino acids and small peptides from the protein-rich substrates such as milk, meat, and vegetables in which they are primarily found.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Exterkate F. A. (1990) Differences in short peptide-substrate cleavage by two cell-envelope-located serine proteinases of Lactococcus lactis subsp.cremoris are related to secondary binding specificity. Appl. Microbiol. Biotechnol. 33, 401–406.

    Article  PubMed  CAS  Google Scholar 

  2. Hull M. E. (1947) Studies on milk proteins. II. Colorimetric determination of the partial hydrolysis of the proteins in milk. J. Dairy Sci. 30, 881–884.

    Article  CAS  Google Scholar 

  3. Church F. C., Swaisgood H. E., Porter D. H. and Catignani G. L. (1983) Spectropho tome tric assay using o-phthaldialdehyde for determination of proteoly-sis in milk and isolated milk proteins.J. Dairy Sci. 66, 1219–1227.

    Article  CAS  Google Scholar 

  4. Twining S. S. (1984) Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal. Biochem. 143, 30–34.

    Article  PubMed  CAS  Google Scholar 

  5. Exterkate F. A., Alting A. C., and Bruinenberg P. G. (1993) Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge characteristics of the substrate-binding region. Appl. Environ. Microbiol. 59, 3640–3647.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Humana Press Inc.

About this protocol

Cite this protocol

de Giori, G.S., Hébert, E.M. (2001). Methods to Determine Proteolytic Activity of Lactic Acid Bacteria. In: Spencer, J.F.T., de Ragout Spencer, A.L. (eds) Food Microbiology Protocols. Methods in Biotechnology, vol 14. Humana Press. https://doi.org/10.1385/1-59259-029-2:197

Download citation

  • DOI: https://doi.org/10.1385/1-59259-029-2:197

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-867-7

  • Online ISBN: 978-1-59259-029-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics