Abstract
Among the probiotic effects attributed to lactic acid bacteria (LAB), the assimilation of cholesterol (1) would be of particular interest for reducing the absorption of dietary cholesterol from the digestive system into the blood. Several studies have indicated that the cholesterol removal would be related to the ability of the cultures to deconjugate bile salts (2).
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References
Hepner G., Fried R., Jeor S., Fusetti L.,and Morin R.(1979) Hypocholesterolemic effect of yogurt and milk. Am. J. Clin. Nutr. 32, 19–24.
Walker D. K. and Gilliland S. E. (1983) Relationship among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus. J. Dairy Sci. 76, 956–961.
Kilara A. (1982) Influence of in vitro gastric digestion on survival of some lactic cultures. Milchwissenschaft 37, 129–132.
De Man J. C., Rogosa M., and Sharpe M. E. (1960) A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130–135.
Rudel L. L. and Morris M. D. (1973). Determination of cholesterol using o-phthalaldehyde. J. Lipid Res. 14, 364.
Dashkevicz M. P. and Feighner S. D. (1988) Development of a differential medium for bile salt hydrolase-active Lactobacillus sp. Appl. Environ. Microbiol. 55, 11–16.
Irvin J. L., Johnson C. G., and Kopalo J. (1944) A photometric method of determination of cholates in bile and blood. J. Biol. Chem. 439–457.
Scalia S. (1988) Simultaneus determination of free and conjugated bile acids in human gastric juice by high performance liquid chromatography. J. Chromatogr. 431, 259–269.
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© 2001 Humana Press Inc.
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de Valdez, G.F., Taranto, M.P. (2001). Probiotic Properties of Lactobacilli. In: Spencer, J.F.T., de Ragout Spencer, A.L. (eds) Food Microbiology Protocols. Methods in Biotechnology, vol 14. Humana Press. https://doi.org/10.1385/1-59259-029-2:173
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DOI: https://doi.org/10.1385/1-59259-029-2:173
Publisher Name: Humana Press
Print ISBN: 978-0-89603-867-7
Online ISBN: 978-1-59259-029-2
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