Abstract
The ability of aqueous two-phase systems to support bioseparations obviously depends intimately on the physicochemical properties of the polymers and yhe phases they form. Measurement of some of these properties can provide insight into the mechanisms responsible for the patition behavior observed. In a more practical sense, measurement of such properties can be used as quality control procedures for industrial separation processes. For instance, if a given separation is to be carried out repetitively and the batch of polymer is o be changed, measurement of the phase diagram and comparing it with what from the previous polymer lot is an obvious requirement. Determination of the interfacial tension might be a preferable procedure for this purpose, however.
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References
Bamberger, S., Brooks, D. E., Sharp, K. A., Van Alstine, J. M., and Webber, T. J. (1985) Preparation of phase sytems and measurement of their physicochemical properties, in Partitioning in Aqueous Two-Phase Systems (Walter, H., Brooks, D. E., and Fisher, D., eds.), Academic Press, Orlando, FL, pp. 85–130.
Brooks, D. E., Sharp, K. A., and Fisher, D. (1985) Theoretical aspects of partitioning, in Partitioning in Aqueous Two-Phase Systems (Walter, H., Brooks, D. E., and Fisher, D., eds.), Academic Press, Orlando, FL, pp. 11–84.
Rotenberg, Y., Boruvka, L., and Neumann, A. W. (1983) Determination of surface tension and contact angle from the shapes of axisymmetric fluid interfaces. J. Colloid Interface Sci. 93, 169–183.
Adamson, A. W. (1976) Physical Chemistry of Surfaces, 3rd ed., Wiley-Interscience, NY, pp. 25–34.
Brooks, D. E., Sharp, K. A., Bamberger, S., Tamblyn, C. H., Seaman, G. V. S., and Walter, H. (1984) Electrostatic and electrokinetic potentials in two polymer aqueous phase systems. J. Colloid Interface Sci. 102, 1–13.
Kortüm, G. (1965) Treatise on Electrochemistry. Elsevier, Amsterdam.
Baygents, J. C. and Saville, D. A. (1991) Electrophoresis of drops and bubbles. J. Chem. Soc. Faraday Trans. 87, 1883–1898.
Seaman, G. V. F. (1975) Electrokinetic behavior of red cells, in The Red Blood Cell, vol. 2 (Surgenor, D. M., ed.), Academic Press, NY, pp. 1135–1229.
Van Wazer, J. R., Lyons, J. W., Kim, K. Y., and Colwell, R. E. (1963) Viscosity and Flow Measurements. Interscience, NY, pp. 187–230.
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Brooks, D.E., Jones, R.N. (2000). Measurement of Some Physical Properties of Aqueous Two-Phase Systems. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnology™, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:35
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DOI: https://doi.org/10.1385/1-59259-028-4:35
Publisher Name: Humana Press
Print ISBN: 978-0-89603-541-6
Online ISBN: 978-1-59259-028-5
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