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Optimization of Extractions in Aqueous Two-Phase Systems

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Book cover Aqueous Two-Phase Systems: Methods and Protocols

Part of the book series: Methods in Biotechnology™ ((MIBT,volume 11))

Abstract

The extraction of proteins in aqueous phase systems is one of the most rapid purification methods available, and very useful for separation of cell debris.

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References

  1. Menge, U. (1994) Purification of proteins from cell culture supernatant solutions, in Methods in Enzymology, vol. 228 (Walter, H. and Johannson, G., eds.). Academic, San Diego, CA, pp. 617–626.

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  2. Menge, U. (1992) Extraction of lipases in aqueous two-phase systems: the strategy for an optimization, in Frontiers in Bioprocessing II (Todd, P., Sikdar, S., and Bier, M., eds.) ACS-Books, Washington, pp. 331–339.

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  3. Koenig, K., Menge, U., Kieß, M., Wray, V., and Flohé, L. (1997) Convenient isolation and kinetic mechanism of glutathionylspermidine synthetase from Chritidiafasciculata. J. Biol. Chem. 272, 11,908–11,915.

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  4. Albertsson, P-Å. (1985) Partition of Cell Particles and Macromolecules. Wiley, New York.

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  5. Menge, U., Morr, M., Mayr, U., and Kula, M. R. (1983) Purification of human fibroblast interferon by extraction in aqueous two-phase systems. J. Appl. Biochem. 5, 75–90.

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  6. Koenig, K., Anastassiadis, K., and Menge, U., unpublished.

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  7. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.

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Author information

Authors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany

    Ulrich Menge

Authors
  1. Ulrich Menge
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Editor information

Editors and Affiliations

  1. Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Rajni Hatti-Kaul

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© 2000 Humana Press Inc.

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Menge, U. (2000). Optimization of Extractions in Aqueous Two-Phase Systems. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnology™, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:235

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  • DOI: https://doi.org/10.1385/1-59259-028-4:235

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-541-6

  • Online ISBN: 978-1-59259-028-5

  • eBook Packages: Springer Protocols

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