Abstract
This chapter describes the purification of rat liver plasma membranes by affinity partitioning in an aqueous polymer two-phase system using the lectin wheat germ agglutinin (WGA) as affinity ligand. Affinity partitioning is advantageous for conventional membrane fractionation techniques in being highly selective, allowing the rapid and high-yield purification of membranes. In addition, the aqueous polymer environment is gentle to membrane structure and function.
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© 2000 Humana Press Inc.
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Jergil, B., Ekblad, L. (2000). Purification of Plasma Membranes by Affinity Partitioning. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnology™, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:193
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DOI: https://doi.org/10.1385/1-59259-028-4:193
Publisher Name: Humana Press
Print ISBN: 978-0-89603-541-6
Online ISBN: 978-1-59259-028-5
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