Abstract
The phase diagram delineates the potential working area for a particular two-phase system and is a “fingerprint” unique to that system under set conditions of, for example, pH, temperature and salt concentration. Information that can be generated from such a diagram (see Fig. 1) includes: the concentration of phase-forming components necessary to form a system with two phases that are in equilibrium, the subsequent concentration of phase components in the top and bottom phases, and the ratio of phase volumes. Present on the diagram is a binodal curve, which divides a region of component concentrations that will form two immiscible aqueous phases (i.e., above the curve) from those that will form one phase (i.e., at and below the curve). Coordinates for all “potential” systems will lie on a tie-line; the tie-line connects two nodes on the binodal, which represent the final concentration of phase components in the top and bottom phases. Moving along the tie-line coordinates denote systems with differing total compositions and volume ratios, but with the same final concentration of phase components in the top and bottom phases. Also present on the binodal is a critical point; just above this point the composition and volume of both phases are almost equal (as is partitioned material). A theoretical account of the phase diagram can be found in refs. 1-3.
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Kaul, A. (2000). The Phase Diagram. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnology™, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:11
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DOI: https://doi.org/10.1385/1-59259-028-4:11
Publisher Name: Humana Press
Print ISBN: 978-0-89603-541-6
Online ISBN: 978-1-59259-028-5
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