Abstract
One of the areas in which the use of immunocytochemistry has had the greatest impact is in the examination of tissue in the medical pathology laboratory. Immunocytochemistry or actually immunohistochemistry in the pathology laboratory enhances the study of diseased tissue. It is important in studying disease to obtain and process the tissue as quickly as possible. The reason for this is so that the cellular constituents can be preserved as completely as possible. As is the case with any solid piece of tissue, the smaller the piece, the easier it is to preserve the cellular constituents. The varied types of tissue also determine, to an extent, preparation protocols because some types require a more specialized form of preservation than do others. Some specimens, like bone, require many days for proper fixation, whereas other looser connective tissues are preserved in a matter of hours by simple immersion fixation. It should be mentioned, though, that depositing large organs, like whole brains, in buckets of fixative may provide fine cellular detail, but will probably result in the loss of some labile brain proteins desired for study (1). The individual who actually obtains the specimen is an important aspect of tissue processing. This individual needs to remove the sample quickly and, before much autolysis occurs, immediately place the correctly sized (small) sample into the desired fixative.
The opinions or assertions herem represent the personal views of the author, and are not to be construed as official or as representing the views of the Department of the Army or the Department of Defense
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References
Guntern, R., Vallet, P., Bouras, C., and Constantinidis, J. (1989) An improved immunohistostaining procedure for peptides in human brain. Experientia 45, 159–161.
Battifora, H. (1991) Assessment of antigen damage in immunohistochemistry. Am. J Clin. Pathol. 96, 669–671
Logm, G., Schnitt, S, and Dvorak, A. (1987) Rapid microwave fixation of human tissues for light macroscopic immunoperoxtdase identificatton of diagnostrcally useful antigens Lab Invest. 57, 585–591
Baumgartner, W., Dettmger, H., Schmeer, N., and Hoffmerster, E (1988) Evaluation of different fixatives and treatments for immunohistochemical demonstration of Coxiellu burnetii in paraffin-embedded tissues. J. Clin. Microbial. 26, 2044–2047.
Ambrogr, L. P., ed. (1957) Manual of Histologic and Special Staining Techniques Armed Forces Institute of Pathology, Washmgton, DC, p. 33
Taschini, P. and MacDonald, D. (1987) Protease digestion step in immunohrstochemical procedures: Ficin as a substitute for trypsin. Lab Med. 18, 532–536
Stirling, J. (1990) Immuno-and affinity probes for electron microscopy: a review of labeling and preparation techniques. J. Histochem. Cytochem. 38, 145–157
Kitamoto, T., Ogomori, K., Tatetshi, J., and Prusiner, S. (1987) Formic acid pre-treatment enhances immunostaining of cerebral and systemrc amyloids. Lab. Znvest. 57, 230–236.
Shi, S, Key, M., and Kalra, K. (1991) Antigen retrreval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohtstochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39, 741–748.
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© 1994 Humana Press Inc.
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Bratthauer, G.L. (1994). Processing of Tissue Specimens. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Bilogy, vol 34. Humana Press. https://doi.org/10.1385/0-89603285-X:97
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DOI: https://doi.org/10.1385/0-89603285-X:97
Publisher Name: Humana Press
Print ISBN: 978-0-89603-285-9
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