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Indirect Immunofluorescent Labeling of Viable Cells

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Immunocytochemical Methods and Protocols

Part of the book series: Methods in Molecular Bilogy ((MIMB,volume 34))

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Abstract

The combination of the specificity of the antigen-antibody interaction with the exquisite sensitivity of fluorescence detection and quantitation yields one of the most widely applicable analytical tools in cell biology (1). Within the last decade, flow cytometry (FCM) has become an integral part of basic immunological research. Elaboration of this technology has been intensively stimulated by a rapidly growing sophistication in monoclonal antibody technology and vice versa (2).

The opinions or assertions herem represent the personal views of the author, and are not to be construed as official or as representing the views of the Department of the Army or the Department of Defense.

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References

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Author information

Authors and Affiliations

  1. Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC

    Robert E. Cunningham

Authors
  1. Robert E. Cunningham
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Editor information

Editors and Affiliations

  1. The Catholic University of America, Washington, DC

    Lorette C. Javois

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© 1994 Humana Press Inc.

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Cunningham, R.E. (1994). Indirect Immunofluorescent Labeling of Viable Cells. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Bilogy, vol 34. Humana Press. https://doi.org/10.1385/0-89603285-X:229

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  • DOI: https://doi.org/10.1385/0-89603285-X:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-285-9

  • Online ISBN: 978-1-59259-521-1

  • eBook Packages: Springer Protocols

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