Abstract
The extracellular matrix of mammalian tissue is composed of a complex mix of constituitive proteins. This matrix must be broken down to recover single cells effectively for culture and/or staining (1). Tissue dissociation and its affiliated problems were described and defined over 80 years ago by Rous and Jones (2). More recent reviews (3, 4) have revealed newer methods for creating single-cell suspensions. Numerous procedures exist for dissociating solid tumors. They are usually multistep procedures involving one or a combination of mechanical, enzymatic, or chemical manipulations. Ideally, the dissociation protocol is individualized for the tissue of interest and evaluated relative to both optimal and representative cell yield.
The opinions or assertions herem represent the personal views of the author, and are not to be construed as official or as representing the views of the Department of the Army or the Department of Defense.
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© 1994 Humana Press Inc.
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Cunningham, R.E. (1994). Tissue Disaggregation. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Bilogy, vol 34. Humana Press. https://doi.org/10.1385/0-89603285-X:225
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DOI: https://doi.org/10.1385/0-89603285-X:225
Publisher Name: Humana Press
Print ISBN: 978-0-89603-285-9
Online ISBN: 978-1-59259-521-1
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